Dimethomorph induces heart and vascular developmental defects by disrupting thyroid hormone in zebrafish embryos.

IF 6.2 2区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Ecotoxicology and Environmental Safety Pub Date : 2025-01-01 Epub Date: 2024-12-18 DOI:10.1016/j.ecoenv.2024.117413
You Wei, Yunlong Meng, Kun Jia, Weijian Lu, Yushan Huang, Huiqiang Lu
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引用次数: 0

Abstract

Dimethomorph (DMT) is a widely-used selective active fungicide that effectively controls downy mildew, crown rot, and late blight in crops. The extensive application of DMT raises concerns about its ecological impact on non-target organisms in the environment. However, there is limited understanding of the toxicological properties of DMT on these organisms. In this study, we utilized zebrafish as an animal model to assess the toxicity of DMT induced by exposure 5.5-72 hours post-fertilization (hpf). During this period, we monitored and evaluated the development of the zebrafish heart and vascular system. Additionally, embryo samples were collected to perform molecular-level detection of PCNA, oxidative stress, and related genes. The results showed a concentration-dependent decrease in survival rate and hatching rate, shortened body length, slowed heart rate, and pericardial edema, body curvature and reduced eye size as DMT exposure concentration increased. Furthermore, DMT exposure led to impairments in the development of the heart, vascular, along with change in the expression levels of relevant genes. It also caused a decrease in cell proliferation and an increase in oxidative stress levels. Moreover, DMT disrupts the normal development of thyroid follicular cells, leading to a reduction in T3 levels. Thyroid hormone supplementation partially reverses the toxicity induced by DMT, increasing eye size, restoring body length, reducing spine curvature, and reducing pericardial edema. Therefore, we speculate that DMT likely affects the development of zebrafish embryos by disrupting normal thyroid follicle development.

菊霉啉通过破坏斑马鱼胚胎的甲状腺激素诱导心脏和血管发育缺陷。
菊霉啉(Dimethomorph, DMT)是一种广泛使用的选择性活性杀菌剂,可有效防治作物霜霉病、腐冠病和晚疫病。DMT的广泛应用引起了人们对其对环境中非靶生物的生态影响的关注。然而,对DMT对这些生物的毒理学特性的了解有限。在本研究中,我们以斑马鱼为动物模型,评估受精后5.5-72 小时(hpf)暴露诱导的DMT毒性。在此期间,我们监测和评估了斑马鱼心脏和血管系统的发育。此外,收集胚胎样本进行PCNA、氧化应激和相关基因的分子水平检测。结果显示,随着DMT暴露浓度的增加,成活率和孵化率呈浓度依赖性下降,体长缩短,心率减慢,心包水肿,体曲率和眼睛大小减小。此外,DMT暴露导致心脏、血管发育受损,同时相关基因表达水平发生变化。它还导致细胞增殖的减少和氧化应激水平的增加。此外,DMT破坏甲状腺滤泡细胞的正常发育,导致T3水平降低。补充甲状腺激素可以部分逆转DMT引起的毒性,增加眼睛大小,恢复体长,减少脊柱弯曲,减轻心包水肿。因此,我们推测DMT可能通过破坏正常的甲状腺滤泡发育来影响斑马鱼胚胎的发育。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
12.10
自引率
5.90%
发文量
1234
审稿时长
88 days
期刊介绍: Ecotoxicology and Environmental Safety is a multi-disciplinary journal that focuses on understanding the exposure and effects of environmental contamination on organisms including human health. The scope of the journal covers three main themes. The topics within these themes, indicated below, include (but are not limited to) the following: Ecotoxicology、Environmental Chemistry、Environmental Safety etc.
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