Anaerobic co-biodegradation of polyhydroxyalkanoate and swine manure for volatile fatty acid production: The impact of C/N ratios and microbial dynamics.

Abstract

Polyhydroxyalkanoate (PHA) is the important biodegradable plastic, however, biodegradation of PHA waste in anaerobic environments emits more CH₄, a potent greenhouse gas. Bioconversion of PHA waste to useful byproducts - volatile fatty acids (VFAs) is a practical method to upcycle carbon from PHA. In this study, PHA waste was anaerobically co-digested with swine manure (SM) (the typical high nitrogen waste) at different C/N ratios. The results indicate that co-digestion of PHA and SM with a C/N ratio of 32.1 achieved VFA production of 5488 mg COD/L and 0.20 g COD/g VS. No significant differences were found in terms of the highest VFA concentrations between treatments with C/N ratios of 43.4 and 32.1. VFA produciton of 3655 mg COD/L and 0.14 g COD/g VS was achieved at 19 days by adjusting the C/N ratio to 19.2. Four bacteria were identified as dominant microorganisms responsible for converting PHA and SM to VFA.