Soyasaponin Bb/Gelatin-Methacryloyl Hydrogel for Cartilage Inflammation Inhibition

IF 3.7 3区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Yuhan Jiang, Tenghai Li, Bingzhang Liu, Yufeng Tian, Yixin Wang, Tian Li* and Duo Zhang*, 
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Abstract

The main causes of failure for cartilage tissue engineering implants are tissue integration, inflammation, and infection. The development of biomaterials with antiforeign body response (FBR) is of particular importance. Herein, we developed a hydrogel loaded with anti-inflammatory drugs to reduce the inflammatory response that follows implantation. The human chondrocytes were used for in vitro study, and cell-laden hydrogel samples were implanted with the backs of rabbits for in vivo study. Soyasaponin Bb (SsBb) as a traditional Chinese medicine could significantly (P < 0.05) downregulate the expression levels of inflammation-related markers including iNOS, COX-2, and IL-6 in chondrocytes induced by IL-1β through the NF-κB signaling pathway. The in vitro experiments demonstrated that a gelatin-methacryloyl (GelMA) hydrogel loaded with SsBb (SsBb/GelMA) could similarly reduce the gene and protein expression levels of inflammation-related markers (iNOS, COX-2, and IL-6). The in vivo anti-inflammatory effects of the SsBb/GelMA hydrogels were assessed by immunohistochemical staining. The results demonstrated that SsBb/GelMA hydrogels inhibited the inflammatory response and downregulated the expression of the inflammatory cytokine IL-6. Therefore, SsBb/GelMA hydrogels are promising candidates for promoting anti-inflammation and cartilage tissue regeneration of implant surfaces.

大豆皂苷Bb/明胶-甲基丙烯酰水凝胶用于软骨炎症抑制
软骨组织工程植入失败的主要原因是组织整合、炎症和感染。具有抗异物反应(FBR)的生物材料的开发具有特别重要的意义。在此,我们开发了一种含有抗炎药物的水凝胶,以减少植入后的炎症反应。体外研究采用人软骨细胞,体内研究采用兔背植入水凝胶。大豆皂苷Bb (SsBb)作为中药可显著(P <;0.05)通过NF-κB信号通路下调IL-1β诱导的软骨细胞中iNOS、COX-2、IL-6等炎症相关标志物的表达水平。体外实验表明,装载SsBb的明胶-甲基丙烯酰(GelMA)水凝胶(SsBb/GelMA)同样可以降低炎症相关标志物(iNOS, COX-2和IL-6)的基因和蛋白质表达水平。免疫组化染色评价SsBb/GelMA水凝胶的体内抗炎作用。结果表明,SsBb/GelMA水凝胶抑制炎症反应,下调炎症细胞因子IL-6的表达。因此,SsBb/GelMA水凝胶是促进种植体表面抗炎和软骨组织再生的有希望的候选者。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Omega
ACS Omega Chemical Engineering-General Chemical Engineering
CiteScore
6.60
自引率
4.90%
发文量
3945
审稿时长
2.4 months
期刊介绍: ACS Omega is an open-access global publication for scientific articles that describe new findings in chemistry and interfacing areas of science, without any perceived evaluation of immediate impact.
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