Preparation, mechanism, and biological applications of targeting LDs ratiometric fluorescent probes for HClO detection

IF 5.7 2区 化学 Q1 CHEMISTRY, ANALYTICAL
Weiling Yan, Ying Liu, Yuhang Wang, Shizeng Pei, Caihong Zhang, Guomei Zhang, Shaomin Shuang, Chuan Dong
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Abstract

Hypochlorous acid (HClO) is a well-known inflammatory signaling molecule, while lipid droplets (LDs) are dynamic organelles closely related to inflammation. Using organic small-molecule fluorescence imaging technology to target LDs for precise monitoring of HClO is one of the most effective methods for diagnosing inflammation-related diseases. A thorough investigation of how probes detect biological markers and the influencing factors can aid in the design of probe molecules, the selection of high-performance tools, and the accuracy of disease detection. To this end, three fluorescent probes (TPA-B, TPA-BF and TPA-SF) based on triphenylamine (TPA) containing C=N double bonds as potential reaction sites were prepared by condensing triphenylamine thiophene aldehyde (TPA-CHO) with a series of aromatic hydrazides (ArCONHNH2). Their performance in ratiometric detecting HClO by targeting LDs had been systematically studied. The results showed that the Ar- structure in ArCONHNH2 can not only effectively regulate the sensing performance to HClO, but also led to different oxidation products. The key reason for this was the electron cloud density around the C=N bond. Furthermore, the selected probe TPA-B, which demonstrated the best spectral performance, was proficiently utilized for the detection of HClO in cells and zebrafish, as well as the detection of HClO in inflammatory diseases. This study compared the oxidation products of C=N bonds by HClO and analyzed the key influencing factors. By integrating spectroscopic and imaging experiments, high-performance diagnostic agents (TPA-B) for inflammation were identified. The approach provided valuable insights into the design of multifunctional probes and opened new pathways for the diagnosis of inflammation-related diseases.

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来源期刊
Analytica Chimica Acta
Analytica Chimica Acta 化学-分析化学
CiteScore
10.40
自引率
6.50%
发文量
1081
审稿时长
38 days
期刊介绍: Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.
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