Soluble Proteins From Conventional and Organic Eggshell Membranes With Different Proteomic Profiles Show Similar In Vitro Biofunctions

IF 3.9 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Qianli Ma, Lya Piaia, Dagnija Loca, Kristaps Rubenis, Janis Locs, Bernd Thiede, Ólafur Eysteinn Sigurjónsson, Håvard Jostein Haugen
{"title":"Soluble Proteins From Conventional and Organic Eggshell Membranes With Different Proteomic Profiles Show Similar In Vitro Biofunctions","authors":"Qianli Ma,&nbsp;Lya Piaia,&nbsp;Dagnija Loca,&nbsp;Kristaps Rubenis,&nbsp;Janis Locs,&nbsp;Bernd Thiede,&nbsp;Ólafur Eysteinn Sigurjónsson,&nbsp;Håvard Jostein Haugen","doi":"10.1002/jbm.a.37848","DOIUrl":null,"url":null,"abstract":"<p>The eggshell membrane (ESM), resembling the extracellular matrix (ECM), acts as a protective barrier against bacterial invasion and offers various biofunctions due to its porous structure and protein-rich composition, such as ovalbumin, ovotransferrin, collagen, soluble protein, and antimicrobial proteins. However, the structure of ESM primarily comprises disulfide bonds and heterochains, which poses a challenge for protein solubilization/extraction. Therefore, the method of dissolving and extracting bioactive protein components from ESM has significant potential value and importance for exploring the reuse of egg waste and environmental protection. In this study, soluble ESM proteins (SEPs) were extracted from conventional (industrial-fed) and organic (free-grounded) using an acidic 3-mercaptopropionic acid (3-MPA) extraction strategy. FTIR was employed to monitor the chemical changes in the ESM, while LC–MS/MS was used to conduct the proteomic analysis. The biocompatibility and effects of SEP cocktails on ECM synthesis were also investigated. The results indicated that the acidic 3-MPA strategy effectively altered the ESM chemical composition, thereby facilitating SEPs extraction. The SEPs from conventional and organic eggs have different protein profiles but with partial overlapping. SEPs from both sources showed similar desirable biosafety profiles and dose-dependent promotion of osteoblastic (ECM) component synthesis, suggesting that different egg sources may contribute to consistent core biological functions of protein products, they may also introduce different functional priorities.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37848","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomedical materials research. Part A","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jbm.a.37848","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0

Abstract

The eggshell membrane (ESM), resembling the extracellular matrix (ECM), acts as a protective barrier against bacterial invasion and offers various biofunctions due to its porous structure and protein-rich composition, such as ovalbumin, ovotransferrin, collagen, soluble protein, and antimicrobial proteins. However, the structure of ESM primarily comprises disulfide bonds and heterochains, which poses a challenge for protein solubilization/extraction. Therefore, the method of dissolving and extracting bioactive protein components from ESM has significant potential value and importance for exploring the reuse of egg waste and environmental protection. In this study, soluble ESM proteins (SEPs) were extracted from conventional (industrial-fed) and organic (free-grounded) using an acidic 3-mercaptopropionic acid (3-MPA) extraction strategy. FTIR was employed to monitor the chemical changes in the ESM, while LC–MS/MS was used to conduct the proteomic analysis. The biocompatibility and effects of SEP cocktails on ECM synthesis were also investigated. The results indicated that the acidic 3-MPA strategy effectively altered the ESM chemical composition, thereby facilitating SEPs extraction. The SEPs from conventional and organic eggs have different protein profiles but with partial overlapping. SEPs from both sources showed similar desirable biosafety profiles and dose-dependent promotion of osteoblastic (ECM) component synthesis, suggesting that different egg sources may contribute to consistent core biological functions of protein products, they may also introduce different functional priorities.

Abstract Image

求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of biomedical materials research. Part A
Journal of biomedical materials research. Part A 工程技术-材料科学:生物材料
CiteScore
10.40
自引率
2.00%
发文量
135
审稿时长
3.6 months
期刊介绍: The Journal of Biomedical Materials Research Part A is an international, interdisciplinary, English-language publication of original contributions concerning studies of the preparation, performance, and evaluation of biomaterials; the chemical, physical, toxicological, and mechanical behavior of materials in physiological environments; and the response of blood and tissues to biomaterials. The Journal publishes peer-reviewed articles on all relevant biomaterial topics including the science and technology of alloys,polymers, ceramics, and reprocessed animal and human tissues in surgery,dentistry, artificial organs, and other medical devices. The Journal also publishes articles in interdisciplinary areas such as tissue engineering and controlled release technology where biomaterials play a significant role in the performance of the medical device. The Journal of Biomedical Materials Research is the official journal of the Society for Biomaterials (USA), the Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Articles are welcomed from all scientists. Membership in the Society for Biomaterials is not a prerequisite for submission.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信