Mycoremediation of heavy metals by Curvularia lunata from Buckingham Canal, Neelankarai, Chennai.

Abstract

The spread and mobilization of toxic heavy metals in the environment have increased to a harmful level in recent years as a result of the fast industrialization occurring all over the world to meet the demands of a rising population. This research aims to analyze and evaluate the mycoremediation abilities of fungal strains that exhibit tolerance to heavy metals, gathered from water samples at Buckingham Canal, Neelankarai, Chennai. Water samples were examined for heavy metal analysis, and the highest toxic heavy metals, Zn, Pb, Mn, Cu, and Cr, were recorded. Three fungal strains were isolated and named EBPL1000, EBPL1001, and EBPL1002 were selected by primary screening (100 ppm) for further studies. Out of three fungal isolates, EBPL1000 grew in all five heavy metal concentrations and showed 2100 ppm as the highest Maximum Tolerance Concentration toward Lead, 2000 ppm tolerance in Zinc and Manganese, 1700 ppm in Chromium, and 1500 ppm in copper, respectively. The fungal isolate EBPL1000 was identified as Curvularia lunata with 100% percentage identity and query coverage. The Biosorption result reveals that lead is the highest biosorbed heavy metal with 79.99% at 100 ppm concentration while copper is the lowest biosorbed with 24.11% heavy metal at 500 ppm concentration. The uptake of Manganese by Curvularia lunata biomass was the highest (5.64 mg/g) of all heavy metal's uptake at 100 ppm concentration. The lowest uptake of heavy metals was copper (0.43 mg/g) at 500 ppm concentration, and the growth profile study under heavy metals stress conditions shows the order of Pb > Mn > Zn > Cr > Cu at 60 h of time intervals at 100 ppm concentration. In addition to the research, FTIR analysis and Molecular Docking studies provide credence to the idea that Curvularia lunata has high biosorption potential and uptake or removal of toxic heavy metals at low cost and in an eco-friendly way from the contaminated environment.