Lizhen Lu, Serge Delrot, Peige Fan, Zhan Zhang, Die Wu, Fengqin Dong, Pedro García-Caparros, Shaohua Li, Zhanwu Dai, Zhenchang Liang
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引用次数: 0
Abstract
Sugar transport plays a pivotal role in determining the productivity of plants and their capacity to act as carbon sinks. In the major fruit crop grapevine (Vitis vinifera L.), the transporter gene V. vinifera Sugars Will Eventually be Exported Transporter 15 (VvSWEET15) is strongly expressed during berry ripening. However, the specific functions of VvSWEET15 and the mechanisms governing its transcriptional regulation remain largely unresolved. Here, we demonstrate that VvSWEET15 functions as a hexose transporter whose expression is associated with the strong sugar accumulation that starts at the véraison stage. We also characterize VvERF105 as a repressor that binds to the LTR-binding element in the VvSWEET15 promoter, thereby downregulating its expression and inhibiting hexose accumulation at the prevéraison stage. In contrast, VvNAC72 is an activator that binds to the VvNAC72-binding domain (CACATG) and promotes VvSWEET15 expression and hexose accumulation at postvéraison stages. Both transcription factors and VvSWEET15 are preferentially expressed in phloem cells. These results demonstrate that the balance between transcriptional activators and repressors is critical in regulating VvSWEET15 expression in sink organs. Further understanding of these processes will help improve plant productivity and their potential to be used as carbon sinks.
糖的运输在决定植物的生产力和它们作为碳汇的能力方面起着关键作用。在主要水果作物葡萄(Vitis vinifera L.)中,转运基因Vitis vinifera Sugars Will final be Exported transporter 15 (VvSWEET15)在浆果成熟过程中强烈表达。然而,VvSWEET15的具体功能及其转录调控机制在很大程度上仍未得到解决。在这里,我们证明了VvSWEET15作为一种己糖转运蛋白的功能,其表达与开始于vvac阶段的强糖积累有关。我们还将VvERF105描述为一种与VvSWEET15启动子中的ltr结合元件结合的抑制因子,从而下调其表达并抑制VvERF105启动子前阶段的己糖积累。相比之下,VvNAC72是一种结合VvNAC72结合域(CACATG)的激活剂,并促进VvSWEET15的表达和vcv后阶段的己糖积累。转录因子和VvSWEET15均优先在韧皮部细胞中表达。这些结果表明,转录激活因子和转录抑制因子之间的平衡对于调节汇器官中VvSWEET15的表达至关重要。进一步了解这些过程将有助于提高植物的生产力和它们作为碳汇的潜力。
期刊介绍:
Title: Plant Cell
Publisher:
Published monthly by the American Society of Plant Biologists (ASPB)
Produced by Sheridan Journal Services, Waterbury, VT
History and Impact:
Established in 1989
Within three years of publication, ranked first in impact among journals in plant sciences
Maintains high standard of excellence
Scope:
Publishes novel research of special significance in plant biology
Focus areas include cellular biology, molecular biology, biochemistry, genetics, development, and evolution
Primary criteria: articles provide new insight of broad interest to plant biologists and are suitable for a wide audience
Tenets:
Publish the most exciting, cutting-edge research in plant cellular and molecular biology
Provide rapid turnaround time for reviewing and publishing research papers
Ensure highest quality reproduction of data
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