AcGLK1 promotes chloroplast division through regulating AcFtsZ1 in Actinidia chinensis.

IF 3.6 3区生物学 Q1 PLANT SCIENCES

Planta Pub Date : 2024-12-18 DOI:10.1007/s00425-024-04592-y

Xue Fang, Lili Zhao, Jian Li, Zhiwen Ma, Feng Zhang, Pengpeng Zheng, Ziyu Wang, Yongsheng Liu, Lihuan Wang

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引用次数: 0

Abstract

Main conclusion: This study unravels a new regulatory member (AcGLK1) that regulates chloroplast division by affecting the expression levels of cytoskeletal filamenting temperature-sensitive Z (FtsZ) in Actinidia chinensis. GOLDEN 2-LIKE (GLK) transcription factor members of GARP subfamily play an irreplaceable role in regulating chloroplast biogenesis and development. Here we report the functional characterization of a novel GLK1 homolog (AcGLK1) isolated from kiwifruit (Actinidia chinensis cultivar 'Hongyang'). Transgenic lines overexpressing AcGLK1 (AcGLK1OE) resulted in an increase of chloroplast number, size and nutrients accumulation in a tomato variety Micro-Tom (Solanum lycopersicum). Transcriptomic data revealed a series of DEGs related to chloroplast division, in which a tomato FtsZ1 homolog (SlFtsZ1) was significantly upregulated in the transgenic lines and could be directly activated by AcGLK1. Furthermore, AcGLK1 was shown to transcriptionally activate expression of kiwifruit FtsZ1 homologous genes (Achv4p23g035689 and Achv4p19g029547) through Y1H and GUS assays. Taken together, we provide evidence showing that AcGLK1 promotes chloroplast division probably through positively regulation of the transcription of FtsZ1 homologs.

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AcGLK1通过调控AcFtsZ1促进猕猴桃叶绿体分裂。

主要结论：本研究揭示了猕猴桃叶绿体分裂的一个新的调控成员AcGLK1，该成员通过影响细胞骨架丝丝温度敏感因子Z （FtsZ）的表达水平来调控叶绿体分裂。GARP亚家族成员中的GLK转录因子在调控叶绿体生物发生和发育中发挥着不可替代的作用。本文报道了从猕猴桃（猕猴桃品种‘红阳’）中分离的一种新的GLK1同源物（AcGLK1）的功能特征。过表达AcGLK1 （AcGLK1OE）的转基因系导致番茄品种微汤姆（Solanum lycopersicum）叶绿体数量、大小和养分积累增加。转录组学数据显示了一系列与叶绿体分裂相关的基因，其中番茄FtsZ1同源物（SlFtsZ1）在转基因株系中显著上调，并可被AcGLK1直接激活。此外，通过Y1H和GUS检测，AcGLK1转录激活了猕猴桃FtsZ1同源基因（Achv4p23g035689和Achv4p19g029547）的表达。综上所述，我们提供的证据表明，AcGLK1可能通过正向调节FtsZ1同源物的转录来促进叶绿体分裂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Planta 生物-植物科学

CiteScore

7.20

自引率

2.30%

发文量

217

审稿时长

2.3 months

期刊介绍： Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.