Carvone-loaded chitosan nanoparticles alleviate joint destruction by downregulating the expression of pro, inflammatory cytokines and MMP-13 in adjuvant-induced rat model.

IF 4.6 2区 医学 Q2 IMMUNOLOGY
Inflammopharmacology Pub Date : 2025-01-01 Epub Date: 2024-12-18 DOI:10.1007/s10787-024-01618-5
Zartashia Kanwal, Bushra Akhtar, Bilal Aslam, Muhammad Imran Arshad
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引用次数: 0

Abstract

Rheumatoid arthritis is an autoimmune illness causing deformity, edema, and joint tenderness. Its long-term treatment burdens the healthcare system and leads to toxicity, and thus, finding safe, effective, and affordable therapies is essential. The current study aimed to exhibit the anti-arthritic activity of Carvone-loaded chitosan nanoparticles to treat Freund's complete adjuvant (FCA) arthritis in rats. Healthy albino rats (n = 35) were distributed into seven groups. The 1st group worked as normal control, while the 2nd was arthritic control. The 3rd group received methotrexate (10 mg/kg/week). The 4th group received Carvone (60 mg/kg/day), while the 5th (30 mg/kg/day), 6th (45 mg/kg/day), and 7th (60 mg/kg/day) groups received Carvone-C-NPs, respectively. Nanoparticles, prepared by the ion gelation method, were characterized by zeta size, potential, scanning electron microscopy, and Fourier transform infrared microscopy. NPs have zeta size (78.82 ± 0.02 nm) and potential (19.96 ± 0.02 mV). A significant reduction was shown in paw swelling (5.52 ± 0.05 mm), arthritic score (2.81 ± 0.23), and rheumatoid factor (14.56 ± 0.68 IU/L) by Carvone-C-NPs. qRT-PCR results showed significant down-regulation of pro-inflammatory cytokines [TNF-α (0.25 ± 0.03), IL-1β (0.21 ± 0.06), IL-17a (0.16 ± 0.12), and IL-33 (0.15 ± 0.01)] and up-regulation of anti-inflammatory cytokines [IL-4 (0.85 ± 0.06) and IL-10 (0.66 ± 0.04)] in ankle joint of Carvone-C-NPs treated group. The radiographical and histopathological findings showed reduced pannus formation, joint swelling, and synovial hyperplasia in the Carvone-C-NPs treated group. Overall, it is concluded that Carvone-C-NPs have remarkable anti-arthritic activity and promising anti-inflammatory properties.

香芹酮负载的壳聚糖纳米颗粒通过下调促炎性细胞因子和MMP-13在佐剂诱导大鼠模型中的表达来减轻关节损伤。
类风湿性关节炎是一种自身免疫性疾病,引起畸形、水肿和关节压痛。它的长期治疗给卫生保健系统带来负担,并导致毒性,因此,寻找安全、有效和负担得起的治疗方法至关重要。本研究旨在展示装载香芹酮的壳聚糖纳米颗粒治疗大鼠的完全佐剂(FCA)关节炎的抗关节炎活性。健康白化病大鼠35只,分为7组。第一组为正常对照组,第二组为关节炎对照组。第三组给予甲氨蝶呤(10 mg/kg/周)。第4组给予香芹酮(60 mg/kg/d),第5组(30 mg/kg/d)、第6组(45 mg/kg/d)、第7组(60 mg/kg/d)给予香芹酮- c - nps。采用离子凝胶法制备的纳米颗粒,通过zeta尺寸、电势、扫描电镜和傅里叶变换红外显微镜对其进行了表征。NPs的zeta尺寸为78.82±0.02 nm,电势为19.96±0.02 mV。香薰酮- c - nps显著降低了足部肿胀(5.52±0.05 mm)、关节炎评分(2.81±0.23)和类风湿因子(14.56±0.68 IU/L)。qRT-PCR结果显示,香芹酮- c - nps治疗组踝关节促炎因子TNF-α(0.25±0.03)、IL-1β(0.21±0.06)、IL-17a(0.16±0.12)、IL-33(0.15±0.01)显著下调,抗炎因子IL-4(0.85±0.06)、IL-10(0.66±0.04)显著上调。影像学和组织病理学结果显示,香芹酮- c - nps治疗组的滑膜形成、关节肿胀和滑膜增生减少。综上所述,香芹酮- c - nps具有显著的抗关节炎活性和抗炎作用。
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来源期刊
Inflammopharmacology
Inflammopharmacology IMMUNOLOGYTOXICOLOGY-TOXICOLOGY
CiteScore
8.00
自引率
3.40%
发文量
200
期刊介绍: Inflammopharmacology is the official publication of the Gastrointestinal Section of the International Union of Basic and Clinical Pharmacology (IUPHAR) and the Hungarian Experimental and Clinical Pharmacology Society (HECPS). Inflammopharmacology publishes papers on all aspects of inflammation and its pharmacological control emphasizing comparisons of (a) different inflammatory states, and (b) the actions, therapeutic efficacy and safety of drugs employed in the treatment of inflammatory conditions. The comparative aspects of the types of inflammatory conditions include gastrointestinal disease (e.g. ulcerative colitis, Crohn''s disease), parasitic diseases, toxicological manifestations of the effects of drugs and environmental agents, arthritic conditions, and inflammatory effects of injury or aging on skeletal muscle. The journal has seven main interest areas: -Drug-Disease Interactions - Conditional Pharmacology - i.e. where the condition (disease or stress state) influences the therapeutic response and side (adverse) effects from anti-inflammatory drugs. Mechanisms of drug-disease and drug disease interactions and the role of different stress states -Rheumatology - particular emphasis on methods of measurement of clinical response effects of new agents, adverse effects from anti-rheumatic drugs -Gastroenterology - with particular emphasis on animal and human models, mechanisms of mucosal inflammation and ulceration and effects of novel and established anti-ulcer, anti-inflammatory agents, or antiparasitic agents -Neuro-Inflammation and Pain - model systems, pharmacology of new analgesic agents and mechanisms of neuro-inflammation and pain -Novel drugs, natural products and nutraceuticals - and their effects on inflammatory processes, especially where there are indications of novel modes action compared with conventional drugs e.g. NSAIDs -Muscle-immune interactions during inflammation [...]
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