Antimicrobial properties of copper tetraamine fluoride.

IF 3.1 4区医学 Q2 BIOPHYSICS

Journal of Applied Biomaterials & Functional Materials Pub Date : 2024-01-01 DOI:10.1177/22808000241307803

Veena Wenqing Xu, Iris Xiaoxue Yin, John Yun Niu, Ollie Yiru Yu, Mohammed Zahedul Islam Nizami, Chun-Hung Chu

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引用次数: 0

Abstract

Background: A stable copper tetraamine fluoride (CTF) with low cytotoxicity has been developed for dental use.

Objective: To investigate the antimicrobial effects of CTF against common microbes associated with dental caries and periodontal disease.

Method: The minimum inhibitory concentrations (MIC) and minimum bactericidal/fungicidal concentrations (MBC/MFC) were used to evaluate the antimicrobial effects of CTF against eight common bacteria and one fungus associated with dental caries and periodontal disease. These nine microbes included cariogenic pathogens (Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, Lacticaseibacillus casei, Actinomyces naeslundii and Candida albicans), pulpitis-related bacteria (Enterococcus faecalis) and periodontal disease-related bacteria (Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans). Transmission electron microscopy (TEM) was employed to examine the morphological changes of microbes with and without CTF treatment.

Results: The MIC of CTF against nine microbes ranged from 80 ppm (Lacticaseibacillus casei) to 640 ppm (Candida albicans and Enterococcus faecalis). The MBC/MFC ranged from 320 ppm (Lacticaseibacillus casei) to 2560 ppm (Candida albicans). TEM revealed abnormal curvature of cell membranes, disrupted cell membranes, cytoplasmic clear zone, and cytoplasmic content leakage of the microbes treated with CTF.

Conclusion: CTF has antimicrobial effects against common oral pathogens and presents a promising antimicrobial agent to aid management of dental caries and periodontal disease.

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四胺氟化铜的抗菌性能。

背景：一种稳定的低细胞毒性氟化四胺铜（CTF）已被开发用于牙科。目的：探讨乳酸菌对龋齿和牙周病相关微生物的抑菌作用。方法：采用最小抑菌浓度（MIC）和最小杀菌/杀真菌浓度（MBC/MFC）评价CTF对龋齿和牙周病相关的8种常见细菌和1种真菌的抗菌效果。这9种微生物包括致龋病原体（变形链球菌、sobrin链球菌、嗜酸乳杆菌、干酪乳杆菌、naeslundii放线菌和白色念珠菌）、牙髓炎相关细菌（粪肠球菌）和牙周病相关细菌（牙龈卟啉单胞菌和放线菌杆菌）。采用透射电镜（TEM）观察CTF处理前后微生物的形态学变化。结果：CTF对9种微生物的MIC从80ppm（干酪乳杆菌）到640ppm（白色念珠菌和粪肠球菌）不等。MBC/MFC范围从320 ppm（干酪乳杆菌）到2560 ppm（白色念珠菌）。透射电镜显示CTF处理后的微生物细胞膜出现异常弯曲、细胞膜破裂、细胞质清晰带、细胞质内容物渗漏。结论：CTF对口腔常见病原菌具有抗菌作用，是治疗龋齿和牙周病的一种有前景的抗菌药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL

CiteScore

4.40

自引率

4.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics