Extensive environmental survey of free-living amoebae and their elusive association with Mycobacterium bovis or Mycobacterium avium subsp. paratuberculosis.

IF 3.5 3区 生物学 Q2 MICROBIOLOGY
Amélie Jessu, Thierry Cochard, Mélanie Burtin, Stéphanie Crapart, Vincent Delafont, Ascel Samba-Louaka, Franck Biet, Jean-Louis Moyen, Yann Héchard
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引用次数: 0

Abstract

Free-living amoebae (FLA) are described as environmental reservoirs for some bacteria able to resist their phagocytosis. In the environment, the fate of Mycobacterium bovis (Mbo) and Mycobacterium avium subsp. paratuberculosis (Map), responsible for bovine tuberculosis and paratuberculosis respectively, remains poorly understood and is considered potentially problematic in the eradication and control of these diseases. We hypothesize that FLA may play a role in the persistence of Mbo and Map in the environment. In this study, 90 samples were collected from herds affected by one or both diseases to investigate the diversity of amoeba and their associated bacteria. Metabarcoding analyses revealed that Acanthamoeba, Copromyxa, Naegleria and Vermamoeba were the most represented genera of FLA, with Pseudomonadota being the bacteria most commonly found associated with FLA. Although no Mbo and Map DNA were identified by sequencing, traces were detected by ddPCR, specifically targeting these bacteria. In conclusion, we described a wide diversity of FLA and associated bacteria in this environment. It also suggests that Map and Mbo could be associated, even weakly, with FLA in the environment. However, this needs to be confirmed by detecting a highest amount of DNA and if possible, cultivable Map and/or Mbo associated with these environmental FLA.

据描述,自由生活的变形虫(FLA)是一些能够抵抗其吞噬作用的细菌的环境贮藏库。牛分枝杆菌(Mbo)和副结核分枝杆菌(Map)分别是牛结核病和副结核病的罪魁祸首,但人们对它们在环境中的去向仍然知之甚少,并认为它们在根除和控制这些疾病方面可能存在问题。我们推测,FLA 可能在 Mbo 和 Map 在环境中的持续存在中发挥作用。在这项研究中,我们从受一种或两种疾病影响的牛群中收集了 90 份样本,以调查阿米巴及其相关细菌的多样性。元条码分析表明,阿米巴属(Acanthamoeba)、Copromyxa、Naegleria 和 Vermamoeba 是 FLA 中最具代表性的菌属,而假单胞菌属(Pseudomonadota)则是最常见的与 FLA 相关的细菌。虽然没有通过测序鉴定出 Mbo 和 Map DNA,但通过 ddPCR 检测到了专门针对这些细菌的痕迹。总之,我们描述了 FLA 及其相关细菌在这一环境中的广泛多样性。这也表明,Map 和 Mbo 可能与环境中的 FLA 有关联,即使是弱关联。不过,这还需要通过检测与这些环境中的 FLA 相关的最高 DNA 量以及(如果可能)可培养的 Map 和/或 Mbo 来证实。
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来源期刊
FEMS microbiology ecology
FEMS microbiology ecology 生物-微生物学
CiteScore
7.50
自引率
2.40%
发文量
132
审稿时长
3 months
期刊介绍: FEMS Microbiology Ecology aims to ensure efficient publication of high-quality papers that are original and provide a significant contribution to the understanding of microbial ecology. The journal contains Research Articles and MiniReviews on fundamental aspects of the ecology of microorganisms in natural soil, aquatic and atmospheric habitats, including extreme environments, and in artificial or managed environments. Research papers on pure cultures and in the areas of plant pathology and medical, food or veterinary microbiology will be published where they provide valuable generic information on microbial ecology. Papers can deal with culturable and non-culturable forms of any type of microorganism: bacteria, archaea, filamentous fungi, yeasts, protozoa, cyanobacteria, algae or viruses. In addition, the journal will publish Perspectives, Current Opinion and Controversy Articles, Commentaries and Letters to the Editor on topical issues in microbial ecology. - Application of ecological theory to microbial ecology - Interactions and signalling between microorganisms and with plants and animals - Interactions between microorganisms and their physicochemical enviornment - Microbial aspects of biogeochemical cycles and processes - Microbial community ecology - Phylogenetic and functional diversity of microbial communities - Evolutionary biology of microorganisms
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