Extremely low-frequency electromagnetic fields targeting spleen modifies the populations of immunocytes in the spleen

Abstract

Our study focused on investigating the bioeffects of extremely low-frequency electromagnetic fields (ELF-EMFs) on the immune function of the spleen. We designed an electromagnetic instrument that can locally target on spleen, the spleens of mice were locally exposed to the ELF-EMF (50 Hz, 30 mT) for 14 days (4 h/day). Parallelly, the isolated splenic T cells were exposed to ELF-EMF (50 Hz, 15 mT) for 2 h. After the exposure, the splenocyte showed a reduced apoptosis rate. Among the splenocytes, the CD4⁺ T cells and natural killer cells accumulated, the percentage of B cells decreased. In vitro study demonstrated that ELF-EMF induced the alteration of T cell subsets, showing an increased percentage of CD4⁺ T cells and a decreased percentage of CD8⁺ T cells. Within CD4⁺ T cells, the population of T helper (Th) 17 cells increased, and the population of regulatory T cells (Treg) cells decreased. The enrichment of the nuclear factor (NF)-κB pathway in the splenic T cells was found to be reduced after exposure to ELF-EMF. Our findings suggest that ELF-EMF regulated the immune function of the spleen by changing the proportion of immune cells in the spleen. Specifically, the differentiation of spleen T cells was induced by ELF-EMF toward Th17 cells and inhibited by ELF-EMF into Treg cells. The NF-κB signaling pathway probably accounts for the effects of ELF-EMF on the spleen T cells.