S. V. Kamzolova, V. A. Samoylenko, R. K. Allayarov, A. A. Mironov, Y. N. Lunina, N. E. Vorobyeva, A. A. Shaikin, M. B. Vainshtein
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引用次数: 0
Abstract
Glucose–fructose syrups were studied for the first time as a carbon source for the biosynthesis of citric acid (CA) by the yeast Yarrowia lipolytica. The producer Y. lipolytica VKM Y-2373 was selected, and the growth conditions were optimized (syrup concentration, 30 g/L; pH, 6.0; aeration, 20% of saturation). The study found that the concentration of ammonium sulfate in the medium had a significant impact on CA production by Y. lipolytica. The maximum CA production was observed at a concentration of 2 g/L (NH4)2SO4, while concentrations of 1.5 and 3.0 g/L resulted in a decrease of 12 and 30%, respectively. Under the selected conditions, Y. lipolytica VKM Y-2373 produced 36.7 g/L CA in a medium containing glucose–fructose syrup with 52% glucose and 57.7 g/L CA with syrup containing 92% glucose.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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