Growth of Chlorella vulgaris during Active Immobilisation in Calcium Alginate

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Biochemistry and Microbiology Pub Date : 2024-10-01 DOI:10.1134/S0003683823603062

B. S. de C. Barcellos, M. Gutterres

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引用次数: 0

Abstract

Microalgae immobilisation is an attractive alternative for wastewater treatment. These microorganisms have high nutrient absorption capability, and when immobilised, are easy to apply and collect into the treatment, although the viability of cells and effectiveness of this process under these conditions still needs to be explored. This study aims to evaluate the viability of Chlorella vulgaris cells taken at 3 initial concentrations, C1, 5.93 × 10⁵ ± 8.84 × 10⁴, C2, 1.75 × 10⁶ ± 5.09 × 10⁵ and C3, 2.95 × 10⁷± 9.81 × 10⁵ cells/mL, immobilised in the calcium alginate beads for the nutrients removal in Jaworski’s modified medium. The cell growth of immobilised and suspended cells was analysed by cell counting and chlorophyll content by fluorescence spectroscopy in an excitation-emission matrix. The results revealed that the immobilised microalgae grew and removed nutrients regardless of initial concentration. The most concentrated immobilised C. vulgaris (C3) had the highest concentration of cells, 1.67 × 10⁸ cells/mL, and removed more nutrients (100% of nitrate and 96.33% of phosphate) with chlorophyll fluorescence of C3, 22.99 RFU at the end of experiment.

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小球藻在海藻酸钙活性固定过程中的生长

微藻固定化是一种极具吸引力的废水处理方法。这些微生物具有很强的营养吸收能力，固定化后易于应用和收集到处理过程中，但在这些条件下细胞的活力和这一过程的有效性仍有待探索。本研究旨在评估固定在海藻酸钙珠中的小球藻细胞的活力，这些细胞有三种初始浓度：C1（5.93 × 105 ± 8.84 × 104）、C2（1.75 × 106 ± 5.09 × 105）和 C3（2.95 × 107 ± 9.81 × 105），用于去除 Jaworski 改良培养基中的营养物质。通过细胞计数分析了固定细胞和悬浮细胞的生长情况，并通过激发-发射矩阵中的荧光光谱分析了叶绿素含量。结果表明，无论初始浓度如何，固定化微藻都能生长并去除营养物质。浓度最高的固定化 C. vulgaris（C3）细胞浓度最高，为 1.67 × 108 cells/mL，去除的营养物质也更多（100% 的硝酸盐和 96.33% 的磷酸盐），实验结束时 C3 的叶绿素荧光值为 22.99 RFU。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.