Constructions, Purifications and Applications of DNA-Antibody Conjugates: A Review

IF 4.3 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

ACS Omega Pub Date : 2024-11-22 DOI:10.1021/acsomega.4c0771410.1021/acsomega.4c07714

Tao Wang, Xuelin Wang, Shuhong Luo, Peng Zhang, Na Li, Can Chen, Jianwen Li, Hao Shi, Hua Dong* and Ruo-Pan Huang*,

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Abstract

A DNA-antibody conjugate is a synthetic molecule that combines the unique functions of both an antibody and DNA. With the increased accessibility of commercialized kits, the procedure for constructing conjugates is simplified and the requirement for chemistry background is reduced. As a result, the difficulty of preparing a DNA-antibody conjugate has been significantly lowered. Therefore, the application of DNA-antibody conjugates has attracted more interest in recent years. The most common application of DNA-antibody conjugates is based on the amplifiable property of DNA through PCR. This includes single-conjugate-based immuno-PCR, paired-conjugates-based proximity ligation assay, and proximity extension assay. These methods achieve highly sensitive or specific detection of target proteins. The conjugated single stranded DNA molecules can also specifically hybridize with another strand containing its complementary sequence. This property can be used to selectively bind fluorophore labeled DNA strands, which plays an important role in tissue imaging and spatial omics. All these factors make DNA-antibody conjugates have a broad range of applications in research, diagnosis, and potentially therapy.

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dna -抗体偶联物的构建、纯化及应用综述

DNA-抗体偶联物是一种合成分子，结合了抗体和DNA的独特功能。随着商品化试剂盒的普及，构建共轭物的程序简化了，对化学背景的要求也降低了。因此，制备dna -抗体偶联物的难度大大降低。因此，近年来dna -抗体偶联物的应用越来越受到人们的关注。DNA-抗体缀合物最常见的应用是基于DNA通过PCR扩增的特性。这包括基于单偶联物的免疫pcr，基于成对偶联物的接近连接试验和接近扩展试验。这些方法实现了对目标蛋白的高度敏感或特异性检测。共轭单链DNA分子也可以特异性地与含有其互补序列的另一条链杂交。该特性可用于选择性结合荧光团标记的DNA链，在组织成像和空间组学中发挥重要作用。所有这些因素使得dna -抗体偶联物在研究、诊断和潜在治疗方面具有广泛的应用。

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来源期刊

ACS Omega Chemical Engineering-General Chemical Engineering

CiteScore

6.60

自引率

4.90%

发文量

3945

审稿时长

2.4 months

期刊介绍： ACS Omega is an open-access global publication for scientific articles that describe new findings in chemistry and interfacing areas of science, without any perceived evaluation of immediate impact.