Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix

IF 11.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Advances Pub Date : 2024-12-18 DOI:10.1126/sciadv.adr3530

Christopher J. Hansen, Jackson H. Rogers, Alexus J. Brown, Naoko Boatwright, Shajila Siricilla, Christine M. O’Brien, Sourav Panja, Cameron M. Nichols, Kanchana Devanathan, Benjamin M. Hardy, Mark D. Does, Adam W. Anderson, Bibhash C. Paria, Anita Mahadevan-Jansen, Jeff Reese, Jennifer L. Herington

{"title":"Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix","authors":"Christopher J. Hansen, Jackson H. Rogers, Alexus J. Brown, Naoko Boatwright, Shajila Siricilla, Christine M. O’Brien, Sourav Panja, Cameron M. Nichols, Kanchana Devanathan, Benjamin M. Hardy, Mark D. Does, Adam W. Anderson, Bibhash C. Paria, Anita Mahadevan-Jansen, Jeff Reese, Jennifer L. Herington","doi":"10.1126/sciadv.adr3530","DOIUrl":null,"url":null,"abstract":"The orientation and function of smooth muscle in the cervix may contribute to the important biomechanical properties that change during pregnancy. Thus, this study examined the three-dimensional structure, smooth muscle phenotype, and mechanical and contractile functions of the upper and lower cervix of nongravid (not pregnant) and gravid (pregnant) mice. In gravid cervix, we uncovered region-specific changes in the structure and organization of fiber tracts. We also detected a greater proportion of contractile smooth muscle cells (SMCs), but an equal proportion of synthetic SMCs, in the upper versus lower cervix. Furthermore, we revealed that the lower cervix had infrequent spontaneous contractions, distension had a minimal effect on contractility, and the upper cervix had forceful contractions in response to labor-inducing agents (oxytocin and prostaglandin E 2 ). These findings identify regional differences in cervix contractility related to contractile SMC content and fiber organization, which could be targeted with diagnostic technologies and for therapeutic intervention.","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"30 1","pages":""},"PeriodicalIF":11.7000,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Science Advances","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.1126/sciadv.adr3530","RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

The orientation and function of smooth muscle in the cervix may contribute to the important biomechanical properties that change during pregnancy. Thus, this study examined the three-dimensional structure, smooth muscle phenotype, and mechanical and contractile functions of the upper and lower cervix of nongravid (not pregnant) and gravid (pregnant) mice. In gravid cervix, we uncovered region-specific changes in the structure and organization of fiber tracts. We also detected a greater proportion of contractile smooth muscle cells (SMCs), but an equal proportion of synthetic SMCs, in the upper versus lower cervix. Furthermore, we revealed that the lower cervix had infrequent spontaneous contractions, distension had a minimal effect on contractility, and the upper cervix had forceful contractions in response to labor-inducing agents (oxytocin and prostaglandin E 2 ). These findings identify regional differences in cervix contractility related to contractile SMC content and fiber organization, which could be targeted with diagnostic technologies and for therapeutic intervention.

查看原文本刊更多论文

宫颈平滑肌的方向和功能可能会影响妊娠期间发生变化的重要生物力学特性。因此，本研究检测了非妊娠（未怀孕）和妊娠（怀孕）小鼠子宫颈上部和下部的三维结构、平滑肌表型以及机械和收缩功能。在妊娠期宫颈中，我们发现纤维束的结构和组织发生了区域特异性变化。我们还在子宫颈上部和下部检测到了更大比例的收缩平滑肌细胞（SMC），但合成 SMC 的比例相同。此外，我们还发现宫颈下段的自发性收缩不频繁，宫颈扩张对收缩力的影响微乎其微，而宫颈上段在催产素和前列腺素 E 2 诱导下收缩有力。这些发现确定了宫颈收缩力的区域差异与收缩性 SMC 含量和纤维组织有关，可作为诊断技术和治疗干预的目标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Science Advances 综合性期刊-综合性期刊

CiteScore

21.40

自引率

1.50%

发文量

1937

审稿时长

29 weeks

期刊介绍： Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.