Shuqi Yang, Yue Yuan, Xiongwei Zhang, Aoju Feng, Yaqin Zou, Pan Yao, Danni Ye, Kaiyi Zeng, Xiaolei Zhang, Zhihong Xu
{"title":"Effects of difenoconazole and pyraclostrobin on the development, reproduction, and detoxifying metabolism of Spodoptera frugiperda (J.E. Smith)","authors":"Shuqi Yang, Yue Yuan, Xiongwei Zhang, Aoju Feng, Yaqin Zou, Pan Yao, Danni Ye, Kaiyi Zeng, Xiaolei Zhang, Zhihong Xu","doi":"10.1016/j.cropro.2024.107081","DOIUrl":null,"url":null,"abstract":"<ce:italic>Spodoptera frugiperda</ce:italic> (J.E. Smith) is a major insect pest affecting corn globally. <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic> often coincides with maize diseases, as their damage periods overlap. Consequently, <ce:italic>S. frugiperda</ce:italic> may be exposed to varying types and quantities of these fungicides during the growing season. The aim of this study was to determine the effects of difenoconazole and pyraclostrobin on the biological and physiological traits of <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic>. Treatment with difenoconazole and pyraclostrobin significantly prolonged the pupal development period in the F<ce:inf loc=\"post\">0</ce:inf> generation of <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic> compared to the control group. Additionally, exposure to difenoconazole shortened the development periods of the 1st and 6th instar larvae and reduced the adult preoviposition period (APOP) in the F<ce:inf loc=\"post\">1</ce:inf> generation of <ce:italic>S. frugiperda</ce:italic>. However, it extended the development period of the 4th instar larvae and pupae, as well as overall longevity. The pyraclostrobin treatment group exhibited a shorter development period of 2nd instar larvae, as well as longer longevity, and more eggs/per female in the F<ce:inf loc=\"post\">1</ce:inf> generation of <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic>. Compared to the control group, the fecundity of the offspring of the pyraclostrobin treatment group increased by 68.24%. Furthermore, treatment with difenoconazole and pyraclostrobin significantly increased the intrinsic rate of increase (<ce:italic>r</ce:italic>) and the finite rate of increase (<ce:italic>λ</ce:italic>) in the F<ce:inf loc=\"post\">1</ce:inf> generation of <ce:italic>S. frugiperda</ce:italic>, while significantly reducing the mean generation time (<ce:italic>T</ce:italic>). Additionally, mRNA expression levels of <ce:italic>CYP6AN4</ce:italic>, <ce:italic>CYP340L1</ce:italic>, <ce:italic>CYP321A10</ce:italic>, <ce:italic>CYP341A11</ce:italic>, <ce:italic>GST19</ce:italic>, <ce:italic>GST22</ce:italic>, <ce:italic>CE13059</ce:italic>, <ce:italic>CXE23</ce:italic>, <ce:italic>ABC01998, ABC18240</ce:italic>, and <ce:italic>UGT17742</ce:italic> were significantly elevated in F<ce:inf loc=\"post\">0</ce:inf> individuals from larvae treated with difenoconazole. Meanwhile, the relative expression levels of <ce:italic>CYP6AN4</ce:italic>, <ce:italic>CYP340L1</ce:italic>, <ce:italic>CYP314A1</ce:italic>, <ce:italic>GST19</ce:italic>, <ce:italic>GST22</ce:italic>, <ce:italic>CE13095</ce:italic>, <ce:italic>CXE20</ce:italic>, <ce:italic>ABC01998</ce:italic>, <ce:italic>ABC18240</ce:italic>, <ce:italic>ABC22158</ce:italic>, and <ce:italic>ABC22158</ce:italic> in the larvae (F<ce:inf loc=\"post\">0</ce:inf>) exposed to pyraclostrobin were also elevated. Compared to the control and difenoconazole treatment groups, the expression of the vitellogenin gene (<ce:italic>Vg</ce:italic>) and vitellogenin receptor (<ce:italic>VgR</ce:italic>) was significantly upregulated after pyraclostrobin treatment. The effects of difenoconazole and pyraclostrobin on the biological and reproductive performance of <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic> could aid in optimizing the management strategies for this insect species.","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"90 1","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Protection","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.cropro.2024.107081","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
Spodoptera frugiperda (J.E. Smith) is a major insect pest affecting corn globally. S. frugiperda often coincides with maize diseases, as their damage periods overlap. Consequently, S. frugiperda may be exposed to varying types and quantities of these fungicides during the growing season. The aim of this study was to determine the effects of difenoconazole and pyraclostrobin on the biological and physiological traits of S. frugiperda. Treatment with difenoconazole and pyraclostrobin significantly prolonged the pupal development period in the F0 generation of S. frugiperda compared to the control group. Additionally, exposure to difenoconazole shortened the development periods of the 1st and 6th instar larvae and reduced the adult preoviposition period (APOP) in the F1 generation of S. frugiperda. However, it extended the development period of the 4th instar larvae and pupae, as well as overall longevity. The pyraclostrobin treatment group exhibited a shorter development period of 2nd instar larvae, as well as longer longevity, and more eggs/per female in the F1 generation of S. frugiperda. Compared to the control group, the fecundity of the offspring of the pyraclostrobin treatment group increased by 68.24%. Furthermore, treatment with difenoconazole and pyraclostrobin significantly increased the intrinsic rate of increase (r) and the finite rate of increase (λ) in the F1 generation of S. frugiperda, while significantly reducing the mean generation time (T). Additionally, mRNA expression levels of CYP6AN4, CYP340L1, CYP321A10, CYP341A11, GST19, GST22, CE13059, CXE23, ABC01998, ABC18240, and UGT17742 were significantly elevated in F0 individuals from larvae treated with difenoconazole. Meanwhile, the relative expression levels of CYP6AN4, CYP340L1, CYP314A1, GST19, GST22, CE13095, CXE20, ABC01998, ABC18240, ABC22158, and ABC22158 in the larvae (F0) exposed to pyraclostrobin were also elevated. Compared to the control and difenoconazole treatment groups, the expression of the vitellogenin gene (Vg) and vitellogenin receptor (VgR) was significantly upregulated after pyraclostrobin treatment. The effects of difenoconazole and pyraclostrobin on the biological and reproductive performance of S. frugiperda could aid in optimizing the management strategies for this insect species.
期刊介绍:
The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics:
-Abiotic damage-
Agronomic control methods-
Assessment of pest and disease damage-
Molecular methods for the detection and assessment of pests and diseases-
Biological control-
Biorational pesticides-
Control of animal pests of world crops-
Control of diseases of crop plants caused by microorganisms-
Control of weeds and integrated management-
Economic considerations-
Effects of plant growth regulators-
Environmental benefits of reduced pesticide use-
Environmental effects of pesticides-
Epidemiology of pests and diseases in relation to control-
GM Crops, and genetic engineering applications-
Importance and control of postharvest crop losses-
Integrated control-
Interrelationships and compatibility among different control strategies-
Invasive species as they relate to implications for crop protection-
Pesticide application methods-
Pest management-
Phytobiomes for pest and disease control-
Resistance management-
Sampling and monitoring schemes for diseases, nematodes, pests and weeds.