Lysosome-targeted dual-locked NIR fluorescent probe for visualization of H2S and viscosity in drug-induced liver injury and tumor models

IF 5.7 2区 化学 Q1 CHEMISTRY, ANALYTICAL
Jingcan Qin, Fei Kong, Jie Huang, Bang Xiao, Yun Bian, Chengwei Shao
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引用次数: 0

Abstract

Background

Lysosomes, as an indispensable subcellular organelle have numerous physiological functions closely associated with H2S and viscosity, and accurate assessment of H2S/viscosity fluctuations in lysosomes is essential for gaining a comprehensive understanding of lysosome-related physiological activities and pathological processes. The previous single-response fluorescent probes for either H2S or viscosity alone have the potential to generate "false positive" signals in a complex biological environment. In contrast, dual-locked probes can simultaneously respond to multiple targets simultaneously, which could effectively eliminate this defect. Therefore, it is essential to constructed a lysosome-targeted dual-locked NIR fluorescent probe for imaging H2S and viscosity.

Results

In this study, we developed a lysosome-targeted dual-locked NIR fluorescent probe (LFP-N3) for imaging H2S and viscosity based on an integrated ICT-TICT process. In the presence of both H2S and high viscosity conditions, the azide moiety of LFP-N3 reacts with H2S, resulting in the formation of LFP-NH2 that facilitates the ICT process; high viscosity condition further restricts the chemical bond rotation of LFP-NH2, which suppresses the TICT process. As a result, the fluorescence signal of LFP-N3 is significantly enhanced at 690 nm with a large Stokes shift (190 nm). Cytotoxicity assay and colocalization experiments in living cells indicated LFP-N3 possessed low cytotoxicity and precise lysosome-targeted capability. Moreover, both in vitro and in vivo experiments further validated that the fluorescence signal of LFP-N3 can be triggered by the presence of both H2S and high viscosity in tumor and drug-induced liver injury models.

Significance

The lysosome-targeted dual-locked NIR fluorescent probe has been successfully utilized to imaging H2S and viscosity in vitro and in vivo. Compared with the single-response fluorescent probes, the dual-locked NIR probe (LFP-N3) could effectively mitigate false-positive signals and increase spatial resolution, and has great potential to be developed as a novel diagnostic agent for lysosome-related diseases.

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来源期刊
Analytica Chimica Acta
Analytica Chimica Acta 化学-分析化学
CiteScore
10.40
自引率
6.50%
发文量
1081
审稿时长
38 days
期刊介绍: Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.
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