Tingting Feng , Dan Li , Jiaxue Yang , Ting Yang , Ting Wu , Wenqiang Zhu , Yi Wang , Taozhu Hu , Longjia Yan , Yi Le , Li Liu
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引用次数: 0
Abstract
Fluoride (F−) are common in the environment and are beneficial in moderate amounts but can be harmful at high concentrations, leading to conditions such as fluorosis influenced by reactive oxygen species (ROS), including hypochlorite (ClO−). This study introduces PZ-DT, a novel fluorescent probe with a phenothiazine core, designed for the sequential detection of F− and ClO−. PZ-DT emits orange-red fluorescence with a Stokes shift greater than 200 nm upon F− detection, demonstrating excellent selectivity and sensitivity, boasting a quantum yield of 19.33 % and a detection limit of 0.71 μM. Upon interaction with F−, PZ-DT forms PZ-F, which exhibits a blue shift in fluorescence upon ClO− detection, maintaining a Stokes shift of over 100 nm, a quantum yield of 36.2 %, and a detection limit of 19.97 nM. In serum analysis, PZ-DT shows a linear relationship between fluorescence intensity and F− concentration, enhancing its potential for quantitative analysis. In cellular imaging, PZ-DT highlights F− in the red channel and ClO− in the green and blue channels, allowing clear visualization of F− and ClO− interactions at the cellular level. In live mouse models, PZ-DT facilitates the visualization of F− dynamics and exposure decay, providing insights into its metabolic processes and potential health effects. These characteristics underscore its utility in the early diagnosis of fluoride-related diseases.
期刊介绍:
Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science.
The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments.
Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate.
Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to:
Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences,
Novel experimental techniques or instrumentation for molecular spectroscopy,
Novel theoretical and computational methods,
Novel applications in photochemistry and photobiology,
Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.