Development of a novel fluorescent probe for the Selective and sequential imaging of fluoride and hypochlorite

IF 4.3 2区 化学 Q1 SPECTROSCOPY
Tingting Feng , Dan Li , Jiaxue Yang , Ting Yang , Ting Wu , Wenqiang Zhu , Yi Wang , Taozhu Hu , Longjia Yan , Yi Le , Li Liu
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引用次数: 0

Abstract

Fluoride (F) are common in the environment and are beneficial in moderate amounts but can be harmful at high concentrations, leading to conditions such as fluorosis influenced by reactive oxygen species (ROS), including hypochlorite (ClO). This study introduces PZ-DT, a novel fluorescent probe with a phenothiazine core, designed for the sequential detection of F and ClO. PZ-DT emits orange-red fluorescence with a Stokes shift greater than 200 nm upon F detection, demonstrating excellent selectivity and sensitivity, boasting a quantum yield of 19.33 % and a detection limit of 0.71 μM. Upon interaction with F, PZ-DT forms PZ-F, which exhibits a blue shift in fluorescence upon ClO detection, maintaining a Stokes shift of over 100 nm, a quantum yield of 36.2 %, and a detection limit of 19.97 nM. In serum analysis, PZ-DT shows a linear relationship between fluorescence intensity and F concentration, enhancing its potential for quantitative analysis. In cellular imaging, PZ-DT highlights F in the red channel and ClO in the green and blue channels, allowing clear visualization of F and ClO interactions at the cellular level. In live mouse models, PZ-DT facilitates the visualization of F dynamics and exposure decay, providing insights into its metabolic processes and potential health effects. These characteristics underscore its utility in the early diagnosis of fluoride-related diseases.

Abstract Image

开发用于氟化物和次氯酸盐选择性和顺序成像的新型荧光探针。
氟化物(F-)在环境中很常见,适量的氟化物对人体有益,但高浓度的氟化物会对人体有害,导致受活性氧(ROS)(包括次氯酸盐(ClO-))影响的氟中毒等病症。本研究介绍的 PZ-DT 是一种以吩噻嗪为核心的新型荧光探针,设计用于顺序检测 F- 和 ClO-。在检测 F- 时,PZ-DT 发出的橙红色荧光的斯托克斯偏移大于 200 nm,表现出极佳的选择性和灵敏度,量子产率高达 19.33 %,检测限为 0.71 μM。与 F- 作用后,PZ-DT 会形成 PZ-F,在检测 ClO- 时,PZ-F 的荧光会发生蓝移,保持超过 100 nm 的斯托克斯偏移,量子产率为 36.2 %,检测限为 19.97 nM。在血清分析中,PZ-DT 显示出荧光强度与 F- 浓度之间的线性关系,提高了其定量分析的潜力。在细胞成像中,PZ-DT 在红色通道中突出显示 F-,在绿色和蓝色通道中突出显示 ClO-,从而在细胞水平上清晰显示 F- 和 ClO- 的相互作用。在活体小鼠模型中,PZ-DT 可促进 F- 动态和暴露衰减的可视化,为了解 F- 的代谢过程和潜在的健康影响提供帮助。这些特点强调了它在氟相关疾病早期诊断中的实用性。
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来源期刊
CiteScore
8.40
自引率
11.40%
发文量
1364
审稿时长
40 days
期刊介绍: Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.
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