Intensifying intermittent aeration for optimizing nutrient and hormone removal in vertical-flow constructed wetlands filled with aerated concrete.

Abstract

Operational strategies have been applied in constructed wetlands to optimize the removal of nutrients and hormones that are still a concern in wastewater treatment. The strategy of intensifying intermittent aeration was investigated in two microcosm-scale vertical-flow constructed wetlands (VFCWs) planted with Eichhornia crassipes onto autoclaved aerated concrete (AC) in the removal of nutrients, estrone (E1), 17β-estradiol (E2) and 17α-ethinylestradiol (EE2). CW-1 (2.4 LO₂ min^-1) and CW-2 (1.4 LO₂ min^-1) were fed with synthetic wastewater in sequencing-batch mode (cycles 48-48-72 h) and intermittently aerated for 1 h, followed by 7 h without aeration for 377 days. Combined with the intensification strategy, the use of planted free-floating macrophytes and concrete-based material (emergent) as filtering media stand out as the innovation and originality aspects of this study. Despite the hormone addition, intensifying aeration enhanced the efficiencies since CW-1 achieved the highest removals with 91% COD, 77% TN, 74% TAN, 60% nitrate, and 97% TP in Stage I (no hormone addition) and 90% COD, 80% TN, 93% TAN, 63% nitrate, and 82% TP in Stage II (with hormone addition). CW-1 achieved the highest removal efficiencies of E1 (84%), E2 (95%), and EE2 (73%). Conversely, the efficiencies decreased under the lower aeration rate (in CW-2) for all parameters. Macrophyte uptake and adsorption stood out for TN (>60.25%) and TP (>27.6%) removal as the main mechanisms in the VFCWs. The characteristics of AC favored ion exchange and precipitation, reinforcing the potential of this material as filtering media in VFCWs. Intensification of intermittent aeration combined with hormone addition diverse and riched the microbial community with the presence of Thauera, Lentimicrobium (denitrification), Candidatus Accumulibacter (phosphorus removal), Pseudomonas, Fusibacter, and Azoarcus (EE2 degradation). Intensifying intermittent aeration was an important strategy to enhance the simultaneous removal of nutrients and hormones in the VFCWs under the evaluated operational conditions.