Protective Effects of Adipose Mesenchymal Stem Cell Secretome On Oxidative Stress-Induced Bisphenol-A in Isolated Rat Testes Mitochondria and Sperm Quality.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY

Jornal Brasileiro de Reproducao Assistida Pub Date : 2025-03-12 DOI:10.5935/1518-0557.20240089

Maryam Zohour Soleimani, Layasadat Khorsandi, Yousef Asadi-Fard, Fatemeh Rezaei-Tazangi, Atefeh Ashtari

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Abstract

Objective: This study aimed to explore the potential protective effects of adipose-derived mesenchymal stem cell secretome (ASE) on oxidative stress triggered by Bisphenol-A (BisA) exposure in testicular mitochondria and sperm quality of rats.

Methods: Testicular tissue mitochondria and sperms were exposed to BisA (8 μM) and ASE (50 or 100 μg). ∆Ψm (mitochondrial membrane potential), reactive oxygen species (ROS) levels, antioxidant biomarkers, and sperm parameters were measured.

Results: BisA elevated biomarkers of oxidative stress in mitochondria, while the levels of antioxidant activity and ∆Ψm decreased significantly. BisA harmed the morphology, survival rate, and mobility of the spermatozoids. ASE lowered malondialdehyde contents and ROS generation in the mitochondria, increased ∆Ψm, and reversed sperm quality.

Conclusions: These data indicated that ASE effectively reduced BisA-induced damage to mitochondria and enhanced sperm quality by averting oxidative stress.

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脂肪间充质干细胞分泌组对氧化应激诱导的大鼠睾丸双酚a、线粒体和精子质量的保护作用

目的：探讨脂肪源性间充质干细胞分泌组（ASE）对大鼠睾丸线粒体双酚a （BisA）暴露引发的氧化应激及精子质量的潜在保护作用。方法：睾丸组织线粒体和精子分别暴露于BisA （8 μM）和ASE（50或100 μg）中。∆Ψm（线粒体膜电位）、活性氧（ROS）水平、抗氧化生物标志物和精子参数。结果：BisA升高了线粒体氧化应激的生物标志物，而抗氧化活性和∆Ψm水平显著降低。BisA损害了精子的形态、存活率和活动性。ASE降低线粒体丙二醛含量和ROS生成，增加∆Ψm，逆转精子质量。结论：这些数据表明，ASE通过避免氧化应激，有效地减少了bisa诱导的线粒体损伤，提高了精子质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Jornal Brasileiro de Reproducao Assistida OBSTETRICS & GYNECOLOGY-

CiteScore

3.30

自引率

6.70%

发文量