In Vivo Contactless, Cellular-Resolution Imaging of the Healthy and Pathological Human Limbus With 250-kHz Point-Scanning SD-OCT.

IF 2.6 3区医学 Q2 OPHTHALMOLOGY

Translational Vision Science & Technology Pub Date : 2024-12-02 DOI:10.1167/tvst.13.12.29

Kostadinka Bizheva, Zohreh Hosseinaee, Kirsten Carter, Denise Hileeto, Brian G Ballios, Luigina Sorbara, Hall F Chew

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引用次数: 0

Abstract

Purpose: To demonstrate that high-seed, ultra-high-resolution spectral-domain optical coherence tomography (SD-OCT) technology can image in vivo fine morphological features in the healthy and pathological human limbus.

Methods: A compact, fiberoptic SD-OCT system was developed for imaging the human limbus. It combines ∼1.5-µm isotropic spatial resolution in ocular tissue and an acquisition rate of 250,000 A-scans per second. The imaging probe was outfitted with two microscope objectives to provide flexibility in the choice of wide field of view and extended depth of focus versus high lateral resolution. The clinical potential of the system was evaluated by imaging subjects with limbal stem cell dysfunction (LSCD; n = 4) and healthy controls (n = 6).

Results: Limbus images acquired from the healthy controls showed normal cellular structure of the limbal crypts, palisades of Vogt (POVs), and vasculature of the underlying scleral tissue. Images acquired from the LSCD subjects showed distortions or absence of POVs, invasion of highly scattering conjunctival tissue over the limbal and peripheral corneal epithelium, scarring and thinning of the limbal epithelium, and neovascularization.

Conclusions: The combination of high OCT spatial resolution and rapid image acquisition rate allows for in vivo, contactless, volumetric visualization of fine morphological details that could be beneficial for the precise diagnosis and grading of LSCD, planning of treatment, and evaluation of the effectiveness of the treatment approaches.

Translational relevance: The OCT technology described here could improve the clinical diagnostics and grading of LSCD, preoperative planning, and postoperative evaluation of LSCD subjects, in addition to monitoring the effectiveness of various LSCD treatments.

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来源期刊

Translational Vision Science & Technology Engineering-Biomedical Engineering

CiteScore

5.70

自引率

3.30%

发文量

346

审稿时长

25 weeks

期刊介绍： Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO. The journal covers a broad spectrum of work, including but not limited to: Applications of stem cell technology for regenerative medicine, Development of new animal models of human diseases, Tissue bioengineering, Chemical engineering to improve virus-based gene delivery, Nanotechnology for drug delivery, Design and synthesis of artificial extracellular matrices, Development of a true microsurgical operating environment, Refining data analysis algorithms to improve in vivo imaging technology, Results of Phase 1 clinical trials, Reverse translational ("bedside to bench") research. TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.