Enhancing starch levels, granule size and phosphate content in Chlamydomonas reinhardtii through overexpression of ChlreSEX4

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2024-12-15 DOI:10.1016/j.plantsci.2024.112360

Florencia Torresi , Julieta B. Carrillo , Diego F. Gomez-Casati , Maria V. Busi , Mariana Martín

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引用次数: 0

Abstract

Chlamydomonas reinhardtii is a green alga that has been widely used as a model organism for studying various cellular processes, including starch metabolism. In this alga, starch undergoes continuous phosphorylation during its synthesis and degradation. We recently identified and characterized ChlreSEX4 (starch excess 4), a glucan phosphatase from C. reinhardtii, orthologous to Arabidopsis thaliana SEX4, which is able to bind and dephosphorylate amylopectin in vitro. To explore the possibility of manipulating starch phosphorylation levels in C. reinhardtii, we overexpressed the ChlreSEX4 gene in Chlamydomonas and characterized the resulting lines. Results showed a high phosphatase activity in the overexpressing lines, accompanied by an increase in starch content, greater granule size and higher levels of granule-bound phosphate, without changes in triglyceride content. This work allowed us not only to discover a new method to enhance starch accumulation without affecting the lipid content of the alga, but also to obtain a more phosphorylated starch, which would have diverse applications in biotechnology.

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通过过表达ChlreSEX4提高莱茵衣藻淀粉水平、颗粒大小和磷酸盐含量。

莱茵衣藻（Chlamydomonas reinhardtii）是一种绿藻，被广泛用作研究包括淀粉代谢在内的各种细胞过程的模式生物。在这种藻类中，淀粉在合成和降解过程中经历了持续的磷酸化。我们最近鉴定并鉴定了一种来自拟南芥（Arabidopsis thaliana）的葡聚糖磷酸酶ChlreSEX4 (starch excess 4)，它与拟南芥的SEX4同源，能够在体外结合支链淀粉并使其去磷酸化。为了探索控制衣藻淀粉磷酸化水平的可能性，我们在衣藻中过表达了ChlreSEX4基因，并对所产生的品系进行了表征。结果表明，在过表达系中，磷酸酶活性高，淀粉含量增加，颗粒大小和颗粒结合磷酸盐水平较高，甘油三酯含量没有变化。这项工作不仅使我们发现了一种在不影响藻类脂质含量的情况下提高淀粉积累的新方法，而且还获得了一种磷酸化程度更高的淀粉，这将在生物技术中有多种应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.