The physiological and molecular responses of potato tuberization to projected future elevated temperatures.

IF 6.5 1区 生物学 Q1 PLANT SCIENCES
Abigail M Guillemette, Guillian Hernández Casanova, John P Hamilton, Eva Pokorná, Petre I Dobrev, Václav Motyka, Aaron M Rashotte, Courtney P Leisner
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Abstract

Potato (Solanum tuberosum L.) is one of the most important food crops globally and is especially vulnerable to heat stress. However, substantial knowledge gaps remain in our understanding of the developmental mechanisms associated with tuber responses to heat stress. This study used whole-plant physiology, transcriptomics, and phytohormone profiling to elucidate how heat stress affects potato tuber development. When plants were grown in projected future elevated temperature conditions, abscisic acid (ABA) levels decreased in leaf and tuber tissues, whereas rates of leaf carbon assimilation and stomatal conductance were not significantly affected compared to those plants grown in historical temperature conditions. While plants grown in projected future elevated temperature conditions initiated more tubers per plant on average, there was a 66% decrease in mature tubers at final harvest compared to those plants grown in historical temperature conditions. We hypothesize that reduced tuber yields at elevated temperatures are not due to reduced tuber initiation, but due to impaired tuber filling. Transcriptomic analysis detected significant changes in the expression of genes related to ABA response, heat stress and starch biosynthesis. The tuberization repressor genes SELF PRUNING 5G (StSP5G) and CONSTANS-LIKE1 (StCOL1) were differentially expressed in tubers grown in elevated temperatures. Two additional known tuberization genes, IDENTITY OF TUBER 1 (StIT1) and TIMING OF CAB EXPRESSION 1 (StTOC1), displayed distinct expression patterns under elevated temperatures compared to historical temperature conditions but were not differentially expressed. This work highlights potential gene targets and key developmental stages associated with tuberization to develop potatoes with greater heat tolerance.

马铃薯(Solanum tuberosum L.)是全球最重要的粮食作物之一,特别容易受到热胁迫的影响。然而,我们对块茎热胁迫反应相关发育机制的了解仍存在很大的知识差距。本研究利用全植物生理学、转录组学和植物激素分析来阐明热胁迫如何影响马铃薯块茎的发育。当植株在预计的未来高温条件下生长时,叶片和块茎组织中的脱落酸(ABA)水平下降,而叶片碳同化率和气孔导度与在历史温度条件下生长的植株相比没有受到显著影响。虽然在预测的未来温度升高条件下生长的植物平均每株能长出更多块茎,但与在历史温度条件下生长的植物相比,最终收获的成熟块茎减少了 66%。我们推测,温度升高导致块茎产量减少的原因不是块茎萌发减少,而是块茎填充受损。转录组分析发现,与 ABA 响应、热胁迫和淀粉生物合成有关的基因表达发生了显著变化。块茎化抑制基因 SELF PRUNING 5G (StSP5G) 和 CONSTANS-LIKE1 (StCOL1) 在高温下生长的块茎中表达量不同。另外两个已知的块茎化基因 IDENTITY OF TUBER 1 (StIT1) 和 TIMING OF CAB EXPRESSION 1 (StTOC1) 与历史温度条件相比,在高温条件下表现出不同的表达模式,但没有差异表达。这项工作强调了与块茎化相关的潜在基因靶标和关键发育阶段,以开发耐热性更强的马铃薯。
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来源期刊
Plant Physiology
Plant Physiology 生物-植物科学
CiteScore
12.20
自引率
5.40%
发文量
535
审稿时长
2.3 months
期刊介绍: Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.
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