The Influence of CLSM Magnification on the Measured Roughness of Differently Prepared Dental Materials.

IF 3.1 3区 材料科学 Q3 CHEMISTRY, PHYSICAL
Materials Pub Date : 2024-12-05 DOI:10.3390/ma17235954
Martin Rosentritt, Anne Schmutzler, Sebastian Hahnel, Laura Kurzendorfer-Brose
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引用次数: 0

Abstract

This in vitro study investigated how varying magnifications (5×, 10×, 20×, and 50×) using a confocal laser scanning microscope (CLSM) influence the measured surface roughness parameters, Ra/Sa and Rz/Sz, of various materials with two surface treatments. Cylindrical specimens (d ≈ 8 mm, h ≈ 3 mm, n = 10) from titanium, zirconia, glass-ceramic, denture base material, and composite underwent diamond treatment (80 μm; wet) and polishing (#4000; wet; Tegramin-25, Struers, G). The surface roughness parameters (Ra/Sa, Rz/Sz) were measured with a CLSM (VK-100, Keyence, J) at 5×, 10×, 20×, and 50× magnifications. Line roughness (Ra/Rz) was measured along a 1000 μm distance in three parallel lines, while area roughness (Sa/Sz) was evaluated over a 2500 μm × 1900 μm area. The statistical analysis included ANOVA, the Bonferroni post hoc test, and Pearson correlation (SPSS 29, IBM, USA; α = 0.05). Ra/Sa and Rz/Sz showed significant differences (p ≤ 0.001, ANOVA) across magnifications, with values decreasing as magnification increased, highest at 5× and lowest at 50×. Titanium, zirconia, and glass-ceramic showed significant measured roughness values from 5× to 50×. Denture base material and composite had lower measured roughness values, especially after polishing. Line and area roughness varied significantly, indicating that magnification affects measured values. Standardizing magnifications is essential to ensure comparability between studies. A 50× magnification captures more detailed profile information while masking larger defects.

CLSM放大对不同牙体材料粗糙度测量的影响。
本体外实验研究了共聚焦激光扫描显微镜(CLSM)不同的放大倍数(5倍、10倍、20倍和50倍)对两种表面处理下不同材料表面粗糙度参数Ra/Sa和Rz/Sz的影响。钛、氧化锆、玻璃陶瓷、义齿基托材料和复合材料的圆柱形试样(d≈8 mm, h≈3 mm, n = 10)采用金刚石处理(80 μm;湿式)和抛光(#4000;湿的;用CLSM (VK-100, Keyence, J)在5倍、10倍、20倍和50倍倍率下测量表面粗糙度参数(Ra/Sa, Rz/Sz)。在3条平行线上沿1000 μm距离测量直线粗糙度(Ra/Rz),在2500 μm × 1900 μm范围内测量区域粗糙度(Sa/Sz)。统计分析包括方差分析、Bonferroni事后检验和Pearson相关分析(SPSS 29, IBM, USA;α = 0.05)。Ra/Sa和Rz/Sz在不同倍率下差异显著(p≤0.001,方差分析),随倍率的增加而降低,在5倍倍率时最高,在50倍倍率时最低。钛、氧化锆和玻璃陶瓷的测量粗糙度值在5x到50x之间。义齿基托材料和复合材料的测量粗糙度值较低,尤其是抛光后。线和面粗糙度变化显著,表明放大倍数影响测量值。标准化放大倍数对于确保研究之间的可比性至关重要。50倍的放大倍率可以捕获更详细的轮廓信息,同时掩盖更大的缺陷。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Materials
Materials MATERIALS SCIENCE, MULTIDISCIPLINARY-
CiteScore
5.80
自引率
14.70%
发文量
7753
审稿时长
1.2 months
期刊介绍: Materials (ISSN 1996-1944) is an open access journal of related scientific research and technology development. It publishes reviews, regular research papers (articles) and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Materials provides a forum for publishing papers which advance the in-depth understanding of the relationship between the structure, the properties or the functions of all kinds of materials. Chemical syntheses, chemical structures and mechanical, chemical, electronic, magnetic and optical properties and various applications will be considered.
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