Growth, Quality, and Nitrogen Metabolism of Medicago sativa Under Continuous Light from Red-Blue-Green LEDs Responded Better to High Nitrogen Concentrations than Under Red-Blue LEDs.

IF 5.6 2区 生物学
Ren Chen, Yanqi Chen, Kunming Lin, Yiming Ding, Wenke Liu, Shurong Wang
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Abstract

Alfalfa is a widely grown forage with a high crude protein content. Clarifying the interactions between light quality and nitrogen level on yield and nitrogen metabolism can purposely improve alfalfa productivity in plant factories with artificial light (PFAL). In this study, the growth, quality, and nitrogen metabolism of alfalfa grown in PFAL were investigated using three nitrate-nitrogen concentrations (10, 15, and 20 mM, labeled as N10, N15, and N20) and continuous light (CL) with two light qualities (red-blue and red-blue-green light, labeled as RB-C and RBG-C). The results showed that the adaptation performance of alfalfa to nitrogen concentrations differed under red-blue and red-blue-green CL. Plant height, stem diameter, leaf area, yield, Chl a + b, Chl a, Chl b, crude protein contents, and NiR activity under the RB-CN15 treatment were significantly higher than RB-CN10 and RB-CN20 treatments. The RB-CN20 treatment showed morphological damage, such as plant dwarfing and leaf chlorosis, and physiological damage, including the accumulation of proline, H2O2, and MDA. However, the difference was that under red-blue-green CL, the leaf area, yield, and Chl a + b, carotenoid, nitrate, and glutamate contents under RBG-CN20 treatment were significantly higher than in the RBG-CN10 and RBG-CN15 treatments. Meanwhile, the contents of soluble sugar, starch, and cysteine were significantly lower. However, the crude protein content reached 21.15 mg·g-1. The fresh yield, dry yield, stomatal conductance, leaf area, plant height, stem diameter, crude protein, GS, and free amino acids of alfalfa were positively correlated with increased green light. In addition, with the increase in nitrogen concentration, photosynthetic capacity, NiR, and GOGAT activities increased, promoting growth and improving feeding value. The growth, yield, photosynthetic pigments, carbon, nitrogen substances, and enzyme activities of alfalfa were significantly affected by the interaction between nitrogen concentration and light quality, whereas leaf/stem ratio and DPPH had no effect. In conclusion, RB-CN15 and RBG-CN20 are suitable for the production of alfalfa in PFAL, and green light can increase the threshold for the nitrogen concentration adaptation of alfalfa.

红-蓝-绿led连续光照下紫花苜蓿生长、品质及氮代谢对高浓度氮的响应优于红-蓝led。
紫花苜蓿是一种广泛种植的粗蛋白质含量高的饲料。阐明光照质量和氮素水平对产量和氮代谢的相互作用,可以有目的地提高人工光照工厂紫花苜蓿的产量。本研究采用3种硝态氮浓度(10、15和20 mM,分别标记为N10、N15和N20)和2种光质(红蓝光和红蓝绿光,分别标记为RB-C和RBG-C)的连续光(CL),研究了PFAL中紫花苜蓿的生长、品质和氮代谢。结果表明,紫花苜蓿在红蓝和红蓝绿CL下对氮浓度的适应能力不同。RB-CN15处理的株高、茎粗、叶面积、产量、Chl a + b、Chl a、Chl b、粗蛋白质含量和近红外活性均显著高于RB-CN10和RB-CN20处理。RB-CN20处理表现出植株矮化、叶片萎黄等形态损害和脯氨酸、H2O2、MDA积累等生理损害。不同的是,在红蓝绿CL处理下,RBG-CN20处理的叶面积、产量、Chl a + b、类胡萝卜素、硝酸盐和谷氨酸含量均显著高于RBG-CN10和RBG-CN15处理。可溶性糖、淀粉和半胱氨酸含量显著降低。粗蛋白质含量达到21.15 mg·g-1。苜蓿的鲜产量、干产量、气孔导度、叶面积、株高、茎粗、粗蛋白质、GS和游离氨基酸与绿光的增加呈显著正相关。此外,随着氮浓度的增加,光合能力、NiR和GOGAT活性均增加,促进了生长,提高了饲用价值。氮浓度和光质量互作对紫花苜蓿的生长、产量、光合色素、碳、氮物质和酶活性有显著影响,而叶茎比和DPPH无影响。综上所述,RB-CN15和RBG-CN20适合在PFAL条件下生产紫花苜蓿,绿光可提高紫花苜蓿对氮浓度的适应阈值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
自引率
10.70%
发文量
13472
审稿时长
1.7 months
期刊介绍: The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).
文献相关原料
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索莱宝
Cysteine
索莱宝
Glutamate
索莱宝
Ammonium
索莱宝
Proline
索莱宝
Starch
索莱宝
Sucrose
索莱宝
Cysteine
索莱宝
Glutamate
索莱宝
Ammonium
索莱宝
Proline
索莱宝
Starch
索莱宝
Sucrose
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