Stability of Respiratory Syncytial Virus in Nasal Aspirate From Patients Infected With RSV

IF 4.3 4区医学 Q1 INFECTIOUS DISEASES

Influenza and Other Respiratory Viruses Pub Date : 2024-12-16 DOI:10.1111/irv.70058

Atsuko Yamamoto, Yoko Hayasaki-Kajiwara, Takamichi Baba, Saori Okaga, Mayumi Kakui, Takao Shishido

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引用次数: 0

Abstract

Background

Evaluation of infectious virus titer is a challenge for respiratory syncytial virus (RSV) clinical trials because of the labile nature of RSV and rapid loss of infectivity without proper specimen handling. However, there has been no rigorous investigation into RSV stability in clinical specimens.

Methods

RSV stability was investigated by evaluating virus titers and defined as titer variation from baseline within three standard deviations of our titration assay. RSV stability in viral transport medium (VTM) at 4°C and the effect of freezing method on stability were evaluated using RSV-A2 stock. RSV stability in nasal aspirates collected in VTM at 4°C was estimated by regression analysis of virus titers measured at several time points. Stability of these specimens stored at −80°C for 10–15 months after freezing by the method, which maintained RSV-A2 stability, was also assessed.

Results

Three standard deviations were calculated from our titration assay as 0.97 log₁₀ 50% tissue culture infectious dose (TCID₅₀/mL), and RSV stability was defined as variation of virus titer from baseline within 1.0 log₁₀TCID₅₀/mL. RSV-A2 in VTM at 4°C was stable for at least 120 h. Freezing at −80°C negatively affected virus stability, whereas freezing in liquid nitrogen or a dry ice-ethanol bath did not. RSV in nasal aspirates was stable for 2 days at 4°C and for 10–15 months at −80°C after snap freezing.

Conclusions

RSV in nasal aspirates in VTM was estimated to be stable for 2 days at 4°C and for approximately 1 year at −80°C.

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来源期刊

Influenza and Other Respiratory Viruses 医学-病毒学

CiteScore

7.20

自引率

4.50%

发文量

120

审稿时长

6-12 weeks

期刊介绍： Influenza and Other Respiratory Viruses is the official journal of the International Society of Influenza and Other Respiratory Virus Diseases - an independent scientific professional society - dedicated to promoting the prevention, detection, treatment, and control of influenza and other respiratory virus diseases. Influenza and Other Respiratory Viruses is an Open Access journal. Copyright on any research article published by Influenza and Other Respiratory Viruses is retained by the author(s). Authors grant Wiley a license to publish the article and identify itself as the original publisher. Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors, citation details and publisher are identified.