Long-term exposure to bisphenol-A causes oxidative stress-related alterations at the genetic and cellular levels in the mature ovary of adult zebrafish.
Mihir R Tank, Harshad B Patel, Harsh R Patel, Rajkumar S Delvadiya, Urvesh D Patel, Dhaval T Fefar, Juhi M Chauhan
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引用次数: 0
Abstract
Bisphenol-A (BPA) is categorized as a major endocrine-disrupting chemical (EDC) used to manufacture many plastic products. BPA affects reproductive performance and promotes infertility by causing hormonal imbalance, mitochondrial dysfunction, and altered gene expression. The present investigation aimed to evaluate the effects of BPA exposure for 28 days on the activity or level of antioxidant response elements (AREs), mRNA expressions of antioxidant genes, and histomorphological changes in the ovary of adult zebrafish. The adult female zebrafish were randomly divided into four experimental groups, viz. control, vehicle (0.01% ethanol), low dose (BPA: 350 µg/L), and high dose (BPA: 700 µg/L) exposure groups. After BPA exposure in both groups, superoxide dismutase (SOD) activity and total antioxidant capacity (TAC) level were significantly (p < 0.05) decreased in the zebrafish ovary. Whereas, catalase (CAT) activity and malondialdehyde (MDA) level were significantly (p < 0.05) increased in both treatment groups. The sod mRNA expression was significantly (p < 0.05) down-regulated in the high-dose BPA-exposed group. Whereas, cat and nuclear factor erythroid 2-related factor 2 (nrf2) mRNA expressions were significantly (p < 0.05) up-regulated in both BPA-treated groups. Noticeable histomorphological alterations were recorded in the ovary of zebrafish exposed to low and high doses of BPA. The alterations in the activity of ARE, mRNA expressions of antioxidant genes, and histopathological changes suggest that exposure to BPA can cause endocrine disruption and damage to the ovary of adult zebrafish caused by oxidative stress.
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Drug and Chemical Toxicology publishes full-length research papers, review articles and short communications that encompass a broad spectrum of toxicological data surrounding risk assessment and harmful exposure. Manuscripts are considered according to their relevance to the journal.
Topics include both descriptive and mechanics research that illustrates the risk assessment implications of exposure to toxic agents. Examples of suitable topics include toxicological studies, which are structural examinations on the effects of dose, metabolism, and statistical or mechanism-based approaches to risk assessment. New findings and methods, along with safety evaluations, are also acceptable. Special issues may be reserved to publish symposium summaries, reviews in toxicology, and overviews of the practical interpretation and application of toxicological data.
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