Aged mice show a reduction in 5-HT neurons and decreased cellular activation in the dentate gyrus when exposed to acute running.

IF 2.7 3区医学 Q1 ANATOMY & MORPHOLOGY

Brain Structure & Function Pub Date : 2024-12-17 DOI:10.1007/s00429-024-02878-1

Josiane do Nascimento Silva, Bianca Andrade Rodrigues, Elisa Mitiko Kawamoto

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引用次数: 0

Abstract

Serotonin (5-HT) is an important neurotransmitter for cognition and neurogenesis in the dentate gyrus (DG), which occurs via movement stimulation such as physical activity. Brain 5-HT function changes secondary to aging require further investigation. We evaluated whether aged animals would present changes in the number of 5-HT neurons in regions such as the dorsal (DRN) and median (MRN) raphe nuclei and possible changes in the rate of cellular activation in the DG in response to acute running, as a reduction in 5-HT neurons could contribute to a decline in neuronal activation in the DG in response to physical activity in aged mice. This study was conducted on adult (3 months old) and aged (19 months old) male and female mice. Immunohistochemistry, microscopic analysis, and treadmill-running tests were also performed. The data revealed that in aged mice, a reduction in the number of 5-HT neurons in the DRN and MRN of male and female mice was observed. The reduction in the DRN was greater in females. Furthermore, aged animals demonstrate a lower rate of c-Fos labeling in the DG when stimulated by physical exercise. These data indicate that aging may be associated with a reduction in the number of 5-HT neurons in the DRN and MRN, which may lead to a decline in 5-HT availability in the target regions, including the DG. The reduced c-Fos expression in the DG after running in aged mice indicates a decreased response to physical activity, which is potentially linked to serotonergic deficits.

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老年小鼠在急性跑步时表现出5-HT神经元的减少和齿状回细胞活化的减少。

5-羟色胺（5-HT）是齿状回（DG）中认知和神经发生的重要神经递质，通过运动刺激（如体育活动）发生。脑5-羟色胺功能继发于衰老的变化有待进一步研究。我们评估了老龄小鼠中脑背核（DRN）和中脑中核（MRN）等区域的5-HT神经元数量是否会发生变化，以及急性跑步时DG细胞激活率是否会发生变化，因为5-HT神经元的减少可能会导致老年小鼠运动时DG神经元激活的下降。本研究以成年（3月龄）和老年（19月龄）雌雄小鼠为实验对象。免疫组织化学、显微分析和跑步机测试也进行了。数据显示，老龄小鼠雌雄小鼠DRN和MRN中5-HT神经元数量减少。DRN的减少在女性中更大。此外，老年动物在体育锻炼的刺激下，DG中c-Fos标记率较低。这些数据表明，衰老可能与DRN和MRN中5-HT神经元数量减少有关，这可能导致包括DG在内的目标区域5-HT可用性下降。老年小鼠跑步后DG中c-Fos表达降低表明对身体活动的反应降低，这可能与血清素能缺陷有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brain Structure & Function 医学-解剖学与形态学

CiteScore

6.00

自引率

6.50%

发文量

168

审稿时长

8 months

期刊介绍： Brain Structure & Function publishes research that provides insight into brain structure−function relationships. Studies published here integrate data spanning from molecular, cellular, developmental, and systems architecture to the neuroanatomy of behavior and cognitive functions. Manuscripts with focus on the spinal cord or the peripheral nervous system are not accepted for publication. Manuscripts with focus on diseases, animal models of diseases, or disease-related mechanisms are only considered for publication, if the findings provide novel insight into the organization and mechanisms of normal brain structure and function.