Unraveling the degradation mechanism of multiple pyrethroid insecticides by Pseudomonas aeruginosa and its environmental bioremediation potential

IF 10.3 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environment International Pub Date : 2024-12-17 DOI:10.1016/j.envint.2024.109221

Hui Liu, Wen-Juan Chen, Zeling Xu, Shao-Fang Chen, Haoran Song, Yaohua Huang, Kalpana Bhatt, Sandhya Mishra, Mohamed A. Ghorab, Lian-Hui Zhang, Shaohua Chen

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Abstract

Extensive use of pyrethroid insecticides poses significant risks to both ecological ecosystems and human beings. Herein, Pseudomonas aeruginosa PAO1 exhibited exceptional degradation capabilities towards a range of pyrethroid family insecticides including etofenprox, bifenthrin, tetramethrin, D-cypermethrin, allethrin, and permethrin, with a degradation efficiency reaching over 84 % within 36 h (50 mg·L^-1). Strain PAO1 demonstrated effective soil bioremediation by removing etofenprox across different concentrations (25–100 mg·kg⁻¹), with a degradation efficiency over 77 % within 15 days. Additionally, 16S rDNA high-throughput sequencing analysis revealed that introduction of strain PAO1 and etofenprox had a notable impact on the soil microbial community. Strain PAO1 displayed a synergistic effect with local degrading bacteria or flora to degrade etofenprox. UPLC-MS/MS analysis identified 2-(4-ethoxyphenyl) propan-2-ol and 3-phenoxybenzoic acid as the major metabolites of etofenprox biodegradation. A new esterase gene (estA) containing conserved motif (GDSL) and catalytic triad (Ser38, Asp310 and His313) was cloned from strain PAO1. Enzyme activity and gene knockout experiments confirmed the pivotal role of estA in pyrethroid biodegradation. The findings from this study shed a new light on elucidating the degradation mechanism of P. aeruginosa PAO1 and present a useful agent for development of effective pyrethroid bioremediation strategies.

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揭示铜绿假单胞菌降解多种拟除虫菊酯杀虫剂的机制及其环境生物修复潜力

拟除虫菊酯类杀虫剂的广泛使用给生态环境和人类都带来了巨大风险。在本研究中，铜绿假单胞菌 PAO1 对一系列拟除虫菊酯类杀虫剂（包括醚菊酯、联苯菊酯、四氯苯菊酯、D-氯氰菊酯、烯菊酯和氯菊酯）表现出卓越的降解能力，在 36 小时（50 mg-L-1）内降解效率超过 84%。菌株 PAO1 能有效去除不同浓度（25-100 mg-kg-1）的乙烯利，在 15 天内降解效率超过 77%。此外，16S rDNA 高通量测序分析表明，引入 PAO1 菌株和乙草胺对土壤微生物群落有显著影响。UPLC-MS/MS分析发现，2-（4-乙氧基苯基）丙-2-醇和3-苯氧基苯甲酸是乙草胺生物降解的主要代谢产物。从 PAO1 菌株中克隆了一个新的酯酶基因（estA），该基因含有保守基序（GDSL）和催化三元组（Ser38、Asp310 和 His313）。酶活性和基因敲除实验证实了estA在拟除虫菊酯生物降解中的关键作用。该研究结果为阐明铜绿微囊藻 PAO1 的降解机制提供了新的思路，并为开发有效的拟除虫菊酯生物修复策略提供了一种有用的制剂。

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来源期刊

Environment International 环境科学-环境科学

CiteScore

21.90

自引率

3.40%

发文量

734

审稿时长

2.8 months

期刊介绍： Environmental Health publishes manuscripts focusing on critical aspects of environmental and occupational medicine, including studies in toxicology and epidemiology, to illuminate the human health implications of exposure to environmental hazards. The journal adopts an open-access model and practices open peer review. It caters to scientists and practitioners across all environmental science domains, directly or indirectly impacting human health and well-being. With a commitment to enhancing the prevention of environmentally-related health risks, Environmental Health serves as a public health journal for the community and scientists engaged in matters of public health significance concerning the environment.