Efficient production of non-immunogenic, long circular RNAs for high protein yield

IF 26.8 1区医学 Q1 ENGINEERING, BIOMEDICAL

Nature Biomedical Engineering Pub Date : 2024-12-17 DOI:10.1038/s41551-024-01307-2

引用次数: 0

Abstract

Using trans-ribozyme-mediated circularization, we have produced circular RNAs that are not limited by RNA sequence or length and that allow for modifications. The synthesized RNAs increase protein expression more than 7,000-fold.

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高效生产非免疫原性长环状 RNA，提高蛋白质产量

利用反式核酸酶介导的环化技术，我们制备出了不受核糖核酸序列或长度限制并可进行修饰的环状核糖核酸。合成的 RNA 可使蛋白质表达量提高 7000 倍以上。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Biomedical Engineering Medicine-Medicine (miscellaneous)

CiteScore

45.30

自引率

1.10%

发文量

138

期刊介绍： Nature Biomedical Engineering is an online-only monthly journal that was launched in January 2017. It aims to publish original research, reviews, and commentary focusing on applied biomedicine and health technology. The journal targets a diverse audience, including life scientists who are involved in developing experimental or computational systems and methods to enhance our understanding of human physiology. It also covers biomedical researchers and engineers who are engaged in designing or optimizing therapies, assays, devices, or procedures for diagnosing or treating diseases. Additionally, clinicians, who make use of research outputs to evaluate patient health or administer therapy in various clinical settings and healthcare contexts, are also part of the target audience.