First molecular detection of bovine gammaherpesvirus type 4 in dairy cows of Iran and its possible role in endometritis.

IF 1.8 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Research Communications Pub Date : 2024-12-16 DOI:10.1007/s11259-024-10617-4

Mehran Ghaemi, Mohammad Sadeghi, Mojtaba Kafi, Zohreh Pakniat

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引用次数: 0

Abstract

A remarkable percentage of cows in industrialized dairy farms can be affected by uterine diseases including endometritis. Different viral and bacterial agents are causing endometritis in dairy cows including Trueperella pyogenes, and bovine alphaherpesvirus 1. Also, bovine gammaherpesvirus type 4 (BoGHV-4) is responsible for different reproductive disorders. The present study aimed to investigate the role of BoGHV-4 in histopathological and cytological changes of post-partum uterine diseases and its relation to bacterial co-infection. Forty-seven uteri were collected from Holstein dairy cows at least 35 days post-partum. Bacterial culture, cytological, and histopathological studies were conducted on the endometrial samples. The PCR reaction was conducted for the sequencing of the gB gene. PCR examination indicated that from 47 uteri obtained from a slaughterhouse, 13 uteri (27.7%) were positive for BoGHV-4. Statistical analysis revealed a significant relationship between virus presence, and histopathologic and cytologic endometritis (P < 0.05). Also, Escherichia coli and Fusobacterium necrophorum presence was significantly related to BoGHV-4 infection. Vast varieties of other bacterial species were isolated from different samples but the relation between these bacteria and BoGHV-4 was not significant (P > 0.05). Altogether, it can be concluded that BoGHV-4 can play some roles in establishing endometrial inflammation increasing leukocyte infiltration in endometrium, and enhancing bacterial infection in the uterus of dairy cows. This is the first study, which detected the BoGHV-4 in Iranian cows using molecular methods.

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伊朗奶牛γ -疱疹病毒4型的首次分子检测及其在子宫内膜炎中的可能作用。

在工业化的奶牛场中，有相当比例的奶牛会受到子宫内膜炎等子宫疾病的影响。不同的病毒和细菌引起奶牛子宫内膜炎，包括化脓性真佩尔菌和牛甲疱疹病毒1。此外，牛γ -疱疹病毒4型（BoGHV-4）是导致不同生殖障碍的原因。本研究旨在探讨BoGHV-4在产后子宫疾病组织病理和细胞学变化中的作用及其与细菌共感染的关系。从产后至少35天的荷斯坦奶牛中采集子宫47例。对子宫内膜样本进行细菌培养、细胞学和组织病理学研究。采用PCR反应对gB基因进行测序。PCR检测显示，从屠宰场获得的47只子宫中，13只子宫（27.7%）BoGHV-4阳性。统计分析显示病毒的存在与组织病理学和细胞学子宫内膜炎有显著的相关性（p0.05）。综上所述，BoGHV-4在奶牛子宫内膜炎症的建立、子宫内膜白细胞浸润增加、子宫细菌感染增强等方面发挥了一定作用。这是第一次使用分子方法在伊朗奶牛中检测到BoGHV-4。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Research Communications 农林科学-兽医学

CiteScore

2.50

自引率

0.00%

发文量

173

审稿时长

3 months

期刊介绍： Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.