A polyphasic approach in the identification and biochemical characterization of Dunaliella tertiolecta with biodiesel potential from a saltern in Mauritius.

IF 2.3 3区 生物学 Q2 MULTIDISCIPLINARY SCIENCES
PeerJ Pub Date : 2024-12-12 eCollection Date: 2024-01-01 DOI:10.7717/peerj.18325
Kamlesh Ramdhony, Daneshwar Puchooa, Turki Kh Faraj, Abdulwahed Fahad Alrefaei, JunFu Li, Rajesh Jeewon
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引用次数: 0

Abstract

Bioprospecting robust and oleaginous strain is crucial for the commercialization of microalgae-based biodiesel. In this study, a microalgal strain SCH18 was isolated from a solar saltern located in Mauritius. This isolate was identified as Dunaliella tertiolecta based on a polyphasic approach that combined molecular, physiological, and morphological analyses. Furthermore, the effect of different salinities on the biochemical composition and fatty acid profile of this microalga was investigated to explore its potential in producing biodiesel. Results from the growth studies showed that salinity of 1.0 M NaCl was optimal for achieving a high growth rate. Under this salt concentration, the growth rate and the doubling time were calculated as 0.39 ± 0.003 day-1 and 1.79 ± 0.01 days, respectively. In terms of biochemical composition, a substantial amount of carbohydrate (42.02 ± 5.20%), moderate amount of protein (30.35 ± 0.18%) and a low lipid content (17.81 ± 2.4%) were obtained under optimal NaCl concentration. The fatty acid analysis indicated the presence of palmitic acid, stearic acid, palmitoleic acid, oleic acid, linoleic acid, gamma, and alpha-linolenic acids, which are suitable for biodiesel synthesis. The predicted biodiesel properties were in accordance with the standard of ASTM 6751, indicating that the microalgal isolate D. tertiolecta SCH18 is a potential candidate for use in biodiesel production.

采用多相法鉴定毛里求斯盐场中具有生物柴油潜力的邓氏藻(Dunaliella tertiolecta)并确定其生化特征。
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来源期刊
PeerJ
PeerJ MULTIDISCIPLINARY SCIENCES-
CiteScore
4.70
自引率
3.70%
发文量
1665
审稿时长
10 weeks
期刊介绍: PeerJ is an open access peer-reviewed scientific journal covering research in the biological and medical sciences. At PeerJ, authors take out a lifetime publication plan (for as little as $99) which allows them to publish articles in the journal for free, forever. PeerJ has 5 Nobel Prize Winners on the Board; they have won several industry and media awards; and they are widely recognized as being one of the most interesting recent developments in academic publishing.
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