{"title":"Substitution of leucine by glutamate perturbs VopE localization to mitochondria: Lessons from yeast model system","authors":"Nandita Sharma , Kiran Heer , Saumya Raychaudhuri","doi":"10.1016/j.mito.2024.101999","DOIUrl":null,"url":null,"abstract":"<div><div>VopE, a type III effector protein of <em>Vibrio cholerae</em>, modulates host mitochondrial function. Mitochondrial entry of VopE is directly linked with an N-terminal precursor sequence known as the mitochondrial targeting sequence or MTS. MTS of VopE is constituted with 23 amino acids. Earlier studies have shown the importance of leucine residue at position 4 in VopE translocation to mitochondria. In the present study, we have identified another leucine residue at position 15 contributing to the mitochondrial uptake of VopE in the yeast model system. Substitution of leucine<sup>15</sup> with glutamate decreases mitochondrial localization and toxicity of the mutants.</div></div>","PeriodicalId":18606,"journal":{"name":"Mitochondrion","volume":"81 ","pages":"Article 101999"},"PeriodicalIF":3.9000,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mitochondrion","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567724924001570","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
VopE, a type III effector protein of Vibrio cholerae, modulates host mitochondrial function. Mitochondrial entry of VopE is directly linked with an N-terminal precursor sequence known as the mitochondrial targeting sequence or MTS. MTS of VopE is constituted with 23 amino acids. Earlier studies have shown the importance of leucine residue at position 4 in VopE translocation to mitochondria. In the present study, we have identified another leucine residue at position 15 contributing to the mitochondrial uptake of VopE in the yeast model system. Substitution of leucine15 with glutamate decreases mitochondrial localization and toxicity of the mutants.
期刊介绍:
Mitochondrion is a definitive, high profile, peer-reviewed international research journal. The scope of Mitochondrion is broad, reporting on basic science of mitochondria from all organisms and from basic research to pathology and clinical aspects of mitochondrial diseases. The journal welcomes original contributions from investigators working in diverse sub-disciplines such as evolution, biophysics, biochemistry, molecular and cell biology, genetics, pharmacology, toxicology, forensic science, programmed cell death, aging, cancer and clinical features of mitochondrial diseases.