Glucantime and quercetin electrospun nanofiber membranes: Fabrication and their evaluation as dressing for cutaneous leishmaniasis.

IF 1.4 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular and biochemical parasitology Pub Date : 2024-12-14 DOI:10.1016/j.molbiopara.2024.111663

Mehdi Karamian, Esmat Alemzadeh, Ali Abedi, Soudabeh Eshaghi, Meghdad Abdollahpour-Alitappeh, Effat Alemzadeh, Motahareh Mahi-Birjand

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引用次数: 0

Abstract

Cutaneous leishmaniasis is considered as one of the most concerns of the World Health Organization (WHO). The main objective of this study was to use polycaprolactone (PCL) nanofiber scaffolds in order to provide a topical drug delivery system capable of delivering glucantime (glu) and quercetin (qur) to cutaneous leishmaniasis wounds. First, PCL/glu/qur, PCL/glu, and PCL/qur nanofibers were prepared by an electrospinning method followed by characterization through scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (FTIR). Subsequently, we investigated the release of the drugs from nano-scaffolds and anti-promastigote effects. Lastly, the effect of nanobandage was evaluated on 20 female inbred BALB/c mice infected with the parasite. The nanofibers were bead-free and uniform with an average diameter of 224 ± 25 nm and showed a sustained release. Results from in vivo experiments showed that the number of amastigotes and macrophages infected with the parasite and the infiltration of inflammatory cells in mice treated with PCL/qur and PCL/glu/qur nanofibers significantly decreased as compared with those treated with the PCL/glu and PCL nanofibers. Collectively, PCL/glu/qur and PCL/qur nanofibers have promising therapeutic effects in cutaneous leishmaniasis wound healing.

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皮肤利什曼病是世界卫生组织（WHO）最关注的疾病之一。本研究的主要目的是使用聚己内酯（PCL）纳米纤维支架，提供一种能够向皮肤利什曼病伤口输送葡糖酸（glu）和槲皮素（qur）的局部给药系统。首先，我们用电纺丝方法制备了 PCL/glu/qur、PCL/glu 和 PCL/qur 纳米纤维，然后用扫描电子显微镜（SEM）和傅立叶变换红外光谱（FTIR）对其进行了表征。随后，我们研究了纳米支架的药物释放和抗原虫效果。最后，我们在 20 只感染寄生虫的雌性近交系 BALB/c 小鼠身上评估了纳米绷带的效果。纳米纤维无珠且均匀，平均直径为 224±25nm，并显示出持续释放的特性。体内实验结果表明，与使用 PCL/glu 和 PCL 纳米纤维的小鼠相比，使用 PCL/qur 和 PCL/glu/qur 纳米纤维的小鼠感染寄生虫的非膜体和巨噬细胞数量以及炎症细胞浸润显著减少。总之，PCL/glu/qur 和 PCL/qur 纳米纤维在皮肤利什曼病伤口愈合方面具有良好的治疗效果。

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来源期刊

Molecular and biochemical parasitology 医学-寄生虫学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

63 days

期刊介绍： The journal provides a medium for rapid publication of investigations of the molecular biology and biochemistry of parasitic protozoa and helminths and their interactions with both the definitive and intermediate host. The main subject areas covered are: • the structure, biosynthesis, degradation, properties and function of DNA, RNA, proteins, lipids, carbohydrates and small molecular-weight substances • intermediary metabolism and bioenergetics • drug target characterization and the mode of action of antiparasitic drugs • molecular and biochemical aspects of membrane structure and function • host-parasite relationships that focus on the parasite, particularly as related to specific parasite molecules. • analysis of genes and genome structure, function and expression • analysis of variation in parasite populations relevant to genetic exchange, pathogenesis, drug and vaccine target characterization, and drug resistance. • parasite protein trafficking, organelle biogenesis, and cellular structure especially with reference to the roles of specific molecules • parasite programmed cell death, development, and cell division at the molecular level.