Recovery of protein from tofu whey wastewater using protein-based coagulant.

IF 2.2 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES
Environmental Technology Pub Date : 2025-06-01 Epub Date: 2024-12-15 DOI:10.1080/09593330.2024.2439067
Widyarani, Gina Nur Annisa, Dhini Annisa Rahmasari Kanto, Atti Sholihah, Sambas, Yessie Widya Sari, Dani Permana
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Abstract

Tofu whey wastewater is the protein-rich liquid by-product of tofu production that has the potential as a source stream for biobased products. Coagulation can be used to recover protein from tofu whey. Biobased coagulants are alternatives for polymer- and metal-based coagulants, particularly if the precipitate is recovered and used for further processes. The tofu whey coagulation performance of three protein-based coagulants, namely soy protein isolate, Moringa oleifera seed, and isolate of Moringa seed protein, was determined with the jar test method. The pH (4, 5, 7, 9) and protein dosage (33-191 mg-protein/L) were varied. The results were compared with chitosan, as a benchmark for biobased coagulants, with similar pH variation and 100-800 mg-chitosan/L dosage variation. Our experiment showed that the optimum pH for protein coagulation was 4 (the initial pH of the tofu whey). Up to 95% turbidity could be removed, with chitosan shown to be the most effective coagulant. On the other hand, chitosan only removed up to 9% of organics as chemical oxygen demand (COD), while soy protein isolate could remove up to 20%. The highest protein removal was obtained by soy protein isolate (35%) followed by Moringa seed (34%), chitosan (25%), and Moringa seed protein (13%). Meanwhile, coagulation with soy protein isolate could recover 11% protein in the precipitate at 60% protein content. Higher protein removal compared to COD suggests a preference towards protein coagulation compared with other organics. Our results suggest that protein-based materials can be applied to recover proteins from tofu whey.

蛋白基混凝剂回收豆腐乳清废水中的蛋白质。
豆腐乳清废水是豆腐生产中富含蛋白质的液体副产品,具有作为生物基产品来源流的潜力。凝血法可以回收豆腐乳清中的蛋白质。生物基混凝剂是聚合物和金属基混凝剂的替代品,特别是如果沉淀物被回收并用于进一步的工艺。采用罐子试验法测定了大豆分离蛋白、辣木籽和辣木籽蛋白分离物三种蛋白质基凝固剂的豆腐乳清混凝性能。pH(4、5、7、9)和蛋白用量(33 ~ 191 mg-protein/L)不同。结果与壳聚糖作为生物基混凝剂的基准,pH变化相似,投加量变化为100-800 mg-壳聚糖/L。实验表明,蛋白凝固的最佳pH值为4(豆腐乳清的初始pH值)。壳聚糖是最有效的混凝剂,可去除高达95%的浊度。另一方面,壳聚糖只能去除高达9%的化学需氧量(COD),而大豆分离蛋白可以去除高达20%的有机物。大豆分离蛋白的去除率最高(35%),其次是辣木籽(34%)、壳聚糖(25%)和辣木籽蛋白(13%)。在蛋白质含量为60%的情况下,用大豆分离蛋白混凝可回收沉淀中11%的蛋白质。与COD相比,更高的蛋白质去除率表明与其他有机物相比,更倾向于蛋白质凝固。我们的研究结果表明,蛋白质基材料可以应用于豆腐乳清中的蛋白质回收。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Environmental Technology
Environmental Technology 环境科学-环境科学
CiteScore
6.50
自引率
3.60%
发文量
0
审稿时长
4 months
期刊介绍: Environmental Technology is a leading journal for the rapid publication of science and technology papers on a wide range of topics in applied environmental studies, from environmental engineering to environmental biotechnology, the circular economy, municipal and industrial wastewater management, drinking-water treatment, air- and water-pollution control, solid-waste management, industrial hygiene and associated technologies. Environmental Technology is intended to provide rapid publication of new developments in environmental technology. The journal has an international readership with a broad scientific base. Contributions will be accepted from scientists and engineers in industry, government and universities. Accepted manuscripts are generally published within four months. Please note that Environmental Technology does not publish any review papers unless for a specified special issue which is decided by the Editor. Please do submit your review papers to our sister journal Environmental Technology Reviews at http://www.tandfonline.com/toc/tetr20/current
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