Identification and Characterization of a Novel Cytorhabdovirus Associated with Pear (P. communis 'Bartlett') Through RNA-seq.

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Malyaj R Prajapati, Nitika Gupta, Pooja Thapa, Damini Diksha, Susheel Kumar Sharma, Virendra Kumar Baranwal
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引用次数: 0

Abstract

Novel viruses in plants can be detected through transcriptome data mining. In this study, a novel cytorhabdovirus, pear rhabdovirus 1 (PRV-1) was identified through reanalysis of RNA-seq data of pear (P. communis 'Bartlett'). The genomic RNA of PRV-1, with complete coding region, measured 15,628 nucleotides (nts) and encompassed six open reading frames (ORF). Homology analysis of PRV-1 genome showed sequence identity of 33.18-56.75% with the existing cytorhabdovirus sequences. Phylogenetic analysis based on genome sequences showed that PRV-1 clustered in the same clade of cytorhabdoviruses. Based on the sequence demarcation criteria, PRV-1 represents a newly discovered species within the Cytorhabdovirus genus of the Rhabdoviridae family. Identification of a novel virus in pear will enhance our understanding on the diversity of plant cytorhabdoviruses.

通过RNA-seq鉴定梨(P. communis 'Bartlett')相关新型细胞病毒并确定其特征。
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来源期刊
Current Microbiology
Current Microbiology 生物-微生物学
CiteScore
4.80
自引率
3.80%
发文量
380
审稿时长
2.5 months
期刊介绍: Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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