Auxin-dependent post-translational regulation of MONOPTEROS in the Arabidopsis root.

IF 7.5 1区生物学 Q1 CELL BIOLOGY

Cell reports Pub Date : 2024-12-14 DOI:10.1016/j.celrep.2024.115083

Alex Cavalleri, Chiara Astori, Jekaterina Truskina, Mara Cucinotta, Etienne Farcot, Elina Chrysanthou, Xiaocai Xu, Jose M Muino, Kerstin Kaufmann, Martin M Kater, Teva Vernoux, Dolf Weijers, Malcolm J Bennett, Rahul Bhosale, Anthony Bishopp, Lucia Colombo

{"title":"Auxin-dependent post-translational regulation of MONOPTEROS in the Arabidopsis root.","authors":"Alex Cavalleri, Chiara Astori, Jekaterina Truskina, Mara Cucinotta, Etienne Farcot, Elina Chrysanthou, Xiaocai Xu, Jose M Muino, Kerstin Kaufmann, Martin M Kater, Teva Vernoux, Dolf Weijers, Malcolm J Bennett, Rahul Bhosale, Anthony Bishopp, Lucia Colombo","doi":"10.1016/j.celrep.2024.115083","DOIUrl":null,"url":null,"abstract":"<p><p>Auxin plays a pivotal role in plant development by activating AUXIN RESPONSE FACTORs (ARFs). Under low auxin levels, ARF activity is inhibited by interacting with Aux/IAAs. Aux/IAAs are degraded when the cellular auxin concentration increases, causing the release of ARF inhibition. Here, we show that levels of the ARF5/MONOPTEROS (MP) protein are regulated in a cell-type-specific and isoform-dependent manner. We find that the stability of MP isoforms is differentially controlled depending on the auxin level. The canonical MP isoform is degraded by the proteasome in root tissues with low auxin levels. While auxin sharpens the MP localization domain in roots, it does not do so in ovules or embryos. Our research highlights a mechanism for providing spatial control of auxin signaling capacity. Together with recent advances in understanding the tissue-specific expression and post-transcriptional modification of auxin signaling components, these results provide insights into understanding how auxin can elicit so many distinct responses.</p>","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"43 12","pages":"115083"},"PeriodicalIF":7.5000,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell reports","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.celrep.2024.115083","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Auxin plays a pivotal role in plant development by activating AUXIN RESPONSE FACTORs (ARFs). Under low auxin levels, ARF activity is inhibited by interacting with Aux/IAAs. Aux/IAAs are degraded when the cellular auxin concentration increases, causing the release of ARF inhibition. Here, we show that levels of the ARF5/MONOPTEROS (MP) protein are regulated in a cell-type-specific and isoform-dependent manner. We find that the stability of MP isoforms is differentially controlled depending on the auxin level. The canonical MP isoform is degraded by the proteasome in root tissues with low auxin levels. While auxin sharpens the MP localization domain in roots, it does not do so in ovules or embryos. Our research highlights a mechanism for providing spatial control of auxin signaling capacity. Together with recent advances in understanding the tissue-specific expression and post-transcriptional modification of auxin signaling components, these results provide insights into understanding how auxin can elicit so many distinct responses.

查看原文本刊更多论文

Auxin 通过激活 AUXIN RESPONSE FACTORs（ARFs）在植物发育过程中发挥着关键作用。在低浓度的辅助素条件下，ARF 的活性会与 Aux/IAAs 相互作用而受到抑制。当细胞中的植物生长素浓度增加时，Aux/IAAs 会被降解，从而导致 ARF 抑制作用的释放。在这里，我们发现 ARF5/MONOPTEROS（MP）蛋白的水平是以细胞类型特异性和同工酶依赖性的方式调节的。我们发现，MP 同工酶的稳定性受植物生长素水平的控制。在辅助素水平较低的根组织中，典型的MP异构体会被蛋白酶体降解。虽然在根中，辅助素能使 MP 定位域变尖，但在胚珠或胚胎中却不会。我们的研究突显了一种对植物生长素信号能力进行空间控制的机制。结合最近在理解组织特异性表达和转录后修饰辅助素信号成分方面取得的进展，这些结果为理解辅助素如何引起如此多不同的反应提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.