Simultaneous analysis of residual prohibited doping substances in foods using gas chromatography-tandem mass spectrometry†

IF 2.7 3区 化学 Q2 CHEMISTRY, ANALYTICAL
Yejin Lee, Yoeseph Cho, Seongeun Jeon, Yinglan Xu, Kang Mi Lee, Ho Jun Kim, Dong-Woo Lee and Junghyun Son
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Abstract

The continuous consumption of various foods increases the risk of unintentional exposure to residual contaminants. Thus, improving premonitoring procedures to ensure food safety is critical. Herein, a rapid and efficient assay was developed to monitor residual contaminants in food, with a focus on banned doping substances. First, 73 doping compounds, including anabolic agents that can be ingested from food were selected, after which a gas chromatography-tandem mass spectrometry (GC-MS/MS) method was developed for their simultaneous screening. Based on the GC-MS/MS-determined food-matrix characteristics and types, a sample-preparation module was developed to optimize the sample-preparation method. Thereafter, the developed analytical method was validated using representative food matrices, and the results confirmed that the developed method obtained good recoveries (80–123% (limit of quantification: 0.01–20 μg kg−1)). To monitor residual doping substances in commercially available foods, the established method was applied to the analysis of 40 food samples, including meat. Notably, endogenous hormones, such as testosterone, nandrolone, 19-norandrosterone, and 19-noretiocholanolone, were detected in the meat samples, although they did not exceed the maximum residue limits. This approach enables the assessment of potential exposure levels to food-borne endogenous hormones, thereby supporting food safety and preventing unintentional doping incidents in athletes.

Abstract Image

利用气相色谱-串联质谱法同时分析食品中的残留禁用兴奋剂。
持续食用各种食物会增加无意中接触残留污染物的风险。因此,改进预先监测程序以确保食品安全至关重要。本文开发了一种快速有效的检测方法来监测食品中的残留污染物,重点是禁用的兴奋剂物质。首先,选择了73种掺杂化合物,其中包括可从食品中摄取的合成代谢剂,然后建立了气相色谱-串联质谱(GC-MS/MS)方法对其进行同时筛选。基于GC-MS/ ms测定的食品基质特征和类型,建立了制样模块,对制样方法进行优化。采用代表性食品基质对所建立的分析方法进行验证,结果表明所建立的方法回收率为80 ~ 123%(定量限为0.01 ~ 20 μ kg-1)。为了监测市售食品中残留的掺杂物质,将所建立的方法应用于分析40种食品样品,包括肉类。值得注意的是,在肉类样品中检测到内源性激素,如睾酮、诺龙酮、去甲雄酮和去甲胆甾醇酮,尽管它们没有超过最大残留限量。这种方法能够评估食源性内源性激素的潜在暴露水平,从而支持食品安全和防止运动员意外服用兴奋剂事件。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Analytical Methods
Analytical Methods CHEMISTRY, ANALYTICAL-FOOD SCIENCE & TECHNOLOGY
CiteScore
5.10
自引率
3.20%
发文量
569
审稿时长
1.8 months
期刊介绍: Early applied demonstrations of new analytical methods with clear societal impact
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