Silk Sericin/Chitosan Supramolecular Multilayered Thin Films as Sustainable Cytocompatible Nanobiomaterials.

IF 5.5 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Miguel Rosas, Cristiana F V Sousa, Ana Pereira, Adérito J R Amaral, Tamagno Pesqueira, Sónia G Patrício, Sara Fateixa, Helena I S Nogueira, João F Mano, Ana L Oliveira, João Borges
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引用次数: 0

Abstract

Silk sericin (SS) has been widely discarded as a waste by the silk textile industry during the degumming process to obtain fibroin. However, in the past decade, an in-depth understanding of its properties and functions turned it into a high added-value biomaterial for biomedical applications. Herein, we report the molecular design and development of sustainable supramolecular multilayered nanobiomaterials encompassing SS and oppositely charged chitosan (CHT) through a combination of self-assembly and electrostatically driven layer-by-layer (LbL) assembly technology. The successful buildup of SS/CHT multilayered nanobiomaterials was demonstrated by the quartz crystal microbalance with dissipation monitoring and attenuated total reflectance-Fourier transform infrared spectroscopy, and the nanofilms' wettable properties and nanofibrillar-like topography were shown by water contact angle, atomic force microscopy, and scanning electron microscopy. In vitro assays demonstrated the cytocompatibility of the LbL nanofilms toward human primary dermal fibroblasts, holding great promise as biofunctional nanocoatings for drug/therapeutics/cell delivery, tissue engineering, and regenerative medicine.

作为可持续细胞相容性纳米生物材料的丝胶/壳聚糖超分子多层薄膜
蚕丝丝胶(SS)在丝绸纺织业脱胶获得纤维素的过程中被作为废弃物广泛丢弃。然而,在过去的十年中,人们对其特性和功能的深入了解使其成为生物医学应用中的一种高附加值生物材料。在此,我们报告了通过结合自组装和静电驱动逐层组装技术,设计和开发出包含 SS 和带相反电荷壳聚糖(CHT)的可持续超分子多层纳米生物材料。石英晶体微天平耗散监测和衰减全反射-傅里叶变换红外光谱法证明了 SS/CHT 多层纳米生物材料的成功构建,水接触角、原子力显微镜和扫描电子显微镜显示了纳米薄膜的润湿性能和纳米纤维状形貌。体外实验表明,LbL 纳米薄膜对人类原生真皮成纤维细胞具有细胞相容性,有望成为用于药物/治疗/细胞输送、组织工程和再生医学的生物功能纳米涂层。
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来源期刊
Biomacromolecules
Biomacromolecules 化学-高分子科学
CiteScore
10.60
自引率
4.80%
发文量
417
审稿时长
1.6 months
期刊介绍: Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.
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