Mimicking the Bone Extracellular Matrix through a Calcium Phosphate-Containing Thiol-Ene Cross-Linked Gelatin Composite.

IF 5.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules Pub Date : 2024-12-16 DOI:10.1021/acs.biomac.4c01182

Laurens Parmentier, Sophie D'Haese, Louis Van der Meeren, Anna Szabó, Andre G Skirtach, Ruslan I Dmitriev, Janis Locs, Sandra Van Vlierberghe

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引用次数: 0

Abstract

Hydroxyapatite (HAP) and amorphous calcium phosphate (ACP) nanoparticles were incorporated into a thiol-ene clickable gelatin network to elucidate to what extent osteogenic differentiation of human dental pulp- and adipose-derived stem cells (HDPSCs/HASCs) could be further boosted. ACP nanoparticles increased the specific surface area by 23% and reduced the density by 13% while maintaining a comparable particle size (ACP: 25 ± 3 nm; HAP: 27 ± 3 nm). Overall, the incorporation of ceramic nanoparticles did not significantly alter the mechanical properties of the ceramic-containing composites compared to the unsubstituted thiol-ene network. ACP nanoparticles at high concentrations promoted a 21-day osteogenic response in HASCs (72.09 ± 20.20 ng Ca²⁺/ng DNA) comparable to HDPSCs, with the latter showing high calcium production irrespective of the ceramic content (78.45 ± 10.87 ng Ca²⁺/ng DNA), suggesting that the provided cues must be optimized according to the investigated cell type toward a cell-interactive coating application stimulating osteogenesis.

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通过含磷酸钙的硫醇-炔交联明胶复合材料模拟骨细胞外基质

将羟基磷灰石（HAP）和无定形磷酸钙（ACP）纳米颗粒纳入硫醇烯可点击明胶网络，以阐明在多大程度上可进一步促进人牙髓和脂肪来源干细胞（HDPSCs/HASCs）的成骨分化。ACP纳米颗粒的比表面积增加了23%，密度降低了13%，同时保持了相当的颗粒大小（ACP：25 ± 3 nm；HAP：27 ± 3 nm）。总体而言，与未取代的硫醇-烯网络相比，陶瓷纳米颗粒的加入并未显著改变含陶瓷复合材料的机械性能。高浓度的 ACP 纳米粒子促进了 HASCs 21 天的成骨反应（72.09 ± 20.20 ng Ca2+/ng DNA），与 HDPSCs 相当，后者无论陶瓷含量多少都显示出高钙生成（78.45 ± 10.87 ng Ca2+/ng DNA），这表明所提供的线索必须根据所研究的细胞类型进行优化，以实现刺激成骨的细胞互动涂层应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomacromolecules 化学-高分子科学

CiteScore

10.60

自引率

4.80%

发文量

417

审稿时长

1.6 months

期刊介绍： Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.