Genetic Incorporation of a Thioxanthone‐Containing Amino Acid for the Design of Artificial Photoenzymes

IF 16.1 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Kai-Yue Chen, Hui Ming, He-Xiang Wang, Hua-Qi Wang, Zheng Xiang
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Abstract

Genetically encodable photosensitizers allows for the design of artificial photoenzymes to expand the scope of abiological reactions. Here, we report the genetic incorporation of a thioxanthone‐containing amino acid into a protein scaffold using an engineered pyrrolysyl‐tRNA/pyrrolysyl‐tRNA synthetase pair. The designer enzyme was engineered to catalyze a dearomative [2 + 2] cycloaddition reaction in high yields (up to > 99% yield) with excellent enantioselectivity (up to 98:2 e.r.). This work provides a robust and facile method for photoenzyme design and lays the foundation for the development of photoenzymatic reactions.
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来源期刊
CiteScore
26.60
自引率
6.60%
发文量
3549
审稿时长
1.5 months
期刊介绍: Angewandte Chemie, a journal of the German Chemical Society (GDCh), maintains a leading position among scholarly journals in general chemistry with an impressive Impact Factor of 16.6 (2022 Journal Citation Reports, Clarivate, 2023). Published weekly in a reader-friendly format, it features new articles almost every day. Established in 1887, Angewandte Chemie is a prominent chemistry journal, offering a dynamic blend of Review-type articles, Highlights, Communications, and Research Articles on a weekly basis, making it unique in the field.
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