Design of novel dual-emitting ratiometric fluorescence system based on FMT-CDs and MnO2 NS for sensitive isoniazid detection in urine sample

IF 4.3 2区 化学 Q1 SPECTROSCOPY
Xin-Ran Guo, Yan-Xin Guo, Juan Chen, Xin-Yue Chen
{"title":"Design of novel dual-emitting ratiometric fluorescence system based on FMT-CDs and MnO2 NS for sensitive isoniazid detection in urine sample","authors":"Xin-Ran Guo,&nbsp;Yan-Xin Guo,&nbsp;Juan Chen,&nbsp;Xin-Yue Chen","doi":"10.1016/j.saa.2024.125586","DOIUrl":null,"url":null,"abstract":"<div><div>In this work, a simple and sensitive ratiometric fluorescence probe to detect isoniazid (INH) was developed on the basis of carbon dots and MnO<sub>2</sub> nanosheets (MnO<sub>2</sub> NS). Nitrogen-doped carbon dots (FMT-CDs) were synthesized by microwave method using formononetin (FMT) in ammonia water. The oxidase-like activity of MnO<sub>2</sub> NS was utilized to oxidize non-fluorescent <em>o</em>-phenylenediamine (OPD) to 2, 3-diaminophenazine (DAP) with orange fluorescence. It was demonstrated that DAP could effectively quench the fluorescence of FMT-CDs through the inner filtering effect (IFE). Due to the reducing nature of INH, MnO<sub>2</sub> NS was decomposed and thus lost its oxidase-like activity after the addition of INH. Therefore, the production of DAP was reduced, and the fluorescence intensity was decreased, while IFE on FMT-CDs was weakened and the fluorescence of FMT-CDs was recovered. Based on the above principle, a sensitive method for the detection of INH was established based on the fluorescence intensity ratio of the two fluorescent substances, FMT-CDs and DAP, and was successfully applied to the detection of INH in human urine samples. The limit of detection (LOD) could reach to 0.16 μM in human urine samples.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"329 ","pages":"Article 125586"},"PeriodicalIF":4.3000,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1386142524017529","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SPECTROSCOPY","Score":null,"Total":0}
引用次数: 0

Abstract

In this work, a simple and sensitive ratiometric fluorescence probe to detect isoniazid (INH) was developed on the basis of carbon dots and MnO2 nanosheets (MnO2 NS). Nitrogen-doped carbon dots (FMT-CDs) were synthesized by microwave method using formononetin (FMT) in ammonia water. The oxidase-like activity of MnO2 NS was utilized to oxidize non-fluorescent o-phenylenediamine (OPD) to 2, 3-diaminophenazine (DAP) with orange fluorescence. It was demonstrated that DAP could effectively quench the fluorescence of FMT-CDs through the inner filtering effect (IFE). Due to the reducing nature of INH, MnO2 NS was decomposed and thus lost its oxidase-like activity after the addition of INH. Therefore, the production of DAP was reduced, and the fluorescence intensity was decreased, while IFE on FMT-CDs was weakened and the fluorescence of FMT-CDs was recovered. Based on the above principle, a sensitive method for the detection of INH was established based on the fluorescence intensity ratio of the two fluorescent substances, FMT-CDs and DAP, and was successfully applied to the detection of INH in human urine samples. The limit of detection (LOD) could reach to 0.16 μM in human urine samples.

Abstract Image

设计基于 FMT-CDs 和 MnO2 NS 的新型双发射比率荧光系统,用于尿样中异烟肼的灵敏检测。
本研究以碳点和 MnO2 纳米片(MnO2 NS)为基础,开发了一种检测异烟肼(INH)的简单灵敏的比率荧光探针。氮掺杂碳点(FMT-CDs)是利用氨水中的甲萘素(FMT)通过微波法合成的。利用 MnO2 NS 的氧化酶样活性将无荧光的邻苯二胺(OPD)氧化成具有橙色荧光的 2,3-二氨基吩嗪(DAP)。实验证明,DAP 可通过内滤效应(IFE)有效淬灭 FMT-CD 的荧光。由于 INH 具有还原性,加入 INH 后,MnO2 NS 会被分解,从而失去类似氧化酶的活性。因此,DAP 的生成减少,荧光强度降低,而 FMT-CD 上的内滤效应减弱,FMT-CD 的荧光恢复。根据上述原理,建立了基于FMT-CDs和DAP两种荧光物质荧光强度比的INH灵敏检测方法,并成功应用于人体尿样中INH的检测。该方法在人体尿样中的检出限可达0.16 μM。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
8.40
自引率
11.40%
发文量
1364
审稿时长
40 days
期刊介绍: Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.
文献相关原料
公司名称
产品信息
索莱宝
L-lysine
索莱宝
L-lysine
索莱宝
L-lysine
索莱宝
L-lysine
上海源叶
Bovine serum albumin (BSA)
上海源叶
Bovine serum albumin (BSA)
麦克林
pyrazinamide
麦克林
ethambutol
麦克林
rifampicin
麦克林
creatinine
麦克林
Formononetin
麦克林
Formononetin
麦克林
creatinine
麦克林
rifampicin
麦克林
ethambutol
麦克林
pyrazinamide
麦克林
pyrazinamide
麦克林
ethambutol
麦克林
rifampicin
麦克林
creatinine
麦克林
Formononetin
麦克林
pyrazinamide
麦克林
ethambutol
麦克林
rifampicin
麦克林
creatinine
麦克林
Formononetin
百灵威
D-histidine
百灵威
D-histidine
阿拉丁
MnSO4·H2O
阿拉丁
D-alanine
阿拉丁
urea
阿拉丁
urea
阿拉丁
D-alanine
阿拉丁
MnSO4·H2O
阿拉丁
urea
阿拉丁
D-alanine
阿拉丁
MnSO4·H2O
阿拉丁
urea
阿拉丁
D-alanine
阿拉丁
MnSO4·H2O
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信