{"title":"A selenium-based fluorescent sensor for the reversible detection of ClO− and H2S in foods","authors":"Siyi Shen, Maozhong Tian, Wenhui Xia, Jinping Song, Yuzhen Wang, Jiyuan Wei, Xiaohui Wang, Yuehua Yuan, Feng Feng","doi":"10.1016/j.saa.2024.125570","DOIUrl":null,"url":null,"abstract":"<div><div>HClO/ClO<sup>−</sup> and H<sub>2</sub>S are two kinds of momentous biological small molecules in the organism, and the redox balance between them is considerable for the physiological and pathological properties of organisms. Hence, it is very crucial to monitor the redox course between HClO and H<sub>2</sub>S. Herein, a reversible fluorescent sensor (IPSe) for ClO<sup>−</sup> and H<sub>2</sub>S was firstly constructed with the selenium atom as the response site and the dicyanoisophorone as the fluorophore. The sensor IPSe could detect ClO<sup>−</sup> with good selectivity and sensitivity due to the oxidation reaction of the selenium atom triggered by ClO<sup>−</sup>. The recognition of IPSe to hypochlorite induced a hypsochromic shift of the absorption maximum from 420 nm to 380 nm. IPSe exhibited the prominent low detection limit of 55.3 nM for detecting ClO<sup>−</sup>, accompanied by distinct fluorescent attenuation. Moreover, H<sub>2</sub>S could efficiently return the fluorescence of the IPSe solution to the original level by H<sub>2</sub>S reducing selenoxide. The experimental results show that the suggested method has high precision and accuracy for the detection of ClO<sup>−</sup> and H<sub>2</sub>S. The applications in real water samples, beverages and cell imaging verified that the IPSe was capable of monitoring the changes in the concentration of ClO<sup>−</sup>/H<sub>2</sub>S, which indicates that it is of great meaning to survey the biological functions of ClO<sup>−</sup> and H<sub>2</sub>S via IPSe.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"329 ","pages":"Article 125570"},"PeriodicalIF":4.3000,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1386142524017360","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SPECTROSCOPY","Score":null,"Total":0}
引用次数: 0
Abstract
HClO/ClO− and H2S are two kinds of momentous biological small molecules in the organism, and the redox balance between them is considerable for the physiological and pathological properties of organisms. Hence, it is very crucial to monitor the redox course between HClO and H2S. Herein, a reversible fluorescent sensor (IPSe) for ClO− and H2S was firstly constructed with the selenium atom as the response site and the dicyanoisophorone as the fluorophore. The sensor IPSe could detect ClO− with good selectivity and sensitivity due to the oxidation reaction of the selenium atom triggered by ClO−. The recognition of IPSe to hypochlorite induced a hypsochromic shift of the absorption maximum from 420 nm to 380 nm. IPSe exhibited the prominent low detection limit of 55.3 nM for detecting ClO−, accompanied by distinct fluorescent attenuation. Moreover, H2S could efficiently return the fluorescence of the IPSe solution to the original level by H2S reducing selenoxide. The experimental results show that the suggested method has high precision and accuracy for the detection of ClO− and H2S. The applications in real water samples, beverages and cell imaging verified that the IPSe was capable of monitoring the changes in the concentration of ClO−/H2S, which indicates that it is of great meaning to survey the biological functions of ClO− and H2S via IPSe.
期刊介绍:
Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science.
The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments.
Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate.
Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to:
Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences,
Novel experimental techniques or instrumentation for molecular spectroscopy,
Novel theoretical and computational methods,
Novel applications in photochemistry and photobiology,
Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.