Unlocking the role of miR-17: Driving G1-S cell cycle transition in oral tongue cancer through integrated bioinformatics and laboratory analyses.

Archives of oral biology Pub Date : 2024-12-09 DOI:10.1016/j.archoralbio.2024.106160

Ammara Ramzan, Muhammad Usman Rashid, Naila Malkani

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引用次数: 0

Abstract

Objective: This study aims to identify miRNA-mediated regulation of the cell cycle in oral tongue cancer.

Methods: Comprehensive computational analysis was performed on the GEO dataset "GSE168227". DIANA Tool-mir path v.3, STRING, Cytoscape 3.6.0, Enrichr, and TargetScan Human 7.2 were utilized to identify and analyze miRNAs and their targets in oral tongue cancer. The identified miRNA and its target genes were further analyzed in oral tongue cancer patients using qPCR and immunohistochemistry (IHC).

Results: Computational analysis revealed miR-17 as a differentially expressed miRNA in oral tongue cancer. Database analysis indicated potential binding sites of miR-17 for CDKN1A and CCND1 mRNA at 3'-UTR. In oral tongue cancer samples, miR-17, CDKN1A, and CCND1expression were upregulated compared to controls. IHC demonstrated overexpression of p21 and Cyclin D1 across various tumor grades, with predominant cytoplasmic expression of p21 observed in oral tongue cancer samples.

Conclusion: The findings suggest that miR-17 may regulate the G1-S transition of the cell cycle in oral tongue cancer. Further validation and functional studies are warranted to confirm their role as biomarkers.

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揭示 miR-17 的作用：通过综合生物信息学和实验室分析，揭示 miR-17 在口腔舌癌中推动 G1-S 细胞周期转变的作用。

研究目的本研究旨在确定 miRNA 介导的口腔舌癌细胞周期调控：对 GEO 数据集 "GSE168227 "进行了综合计算分析。利用 DIANA Tool-mir path v.3、STRING、Cytoscape 3.6.0、Enrichr 和 TargetScan Human 7.2 来鉴定和分析口腔舌癌中的 miRNA 及其靶标。利用 qPCR 和免疫组化（IHC）技术进一步分析了口腔舌癌患者中已鉴定的 miRNA 及其靶基因：结果：计算分析表明，miR-17 是口腔舌癌中差异表达的 miRNA。数据库分析表明，miR-17 与 CDKN1A 和 CCND1 mRNA 的 3'-UTR 有潜在结合位点。在口腔舌癌样本中，与对照组相比，miR-17、CDKN1A 和 CCND1 表达上调。IHC 显示，p21 和 Cyclin D1 在不同等级的肿瘤中均有过表达，在口腔舌癌样本中，p21 主要在细胞质中表达：结论：研究结果表明，miR-17 可调控口腔舌癌细胞周期的 G1-S 转变。结论：研究结果表明，miR-17 可能会调控口腔舌癌细胞周期的 G1-S 转变，需要进一步的验证和功能研究来确认其作为生物标志物的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Archives of oral biology

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