Culture media with antioxidants improved preimplantation embryo development and clinical outcomes of patients of advanced age.

IF 3.7 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online Pub Date : 2024-08-22 DOI:10.1016/j.rbmo.2024.104415

Shigetoshi Mizumoto, Yozo Nagao, Hitomi Watanabe, Keiko Tanaka, Takeshi Kuramoto

{"title":"Culture media with antioxidants improved preimplantation embryo development and clinical outcomes of patients of advanced age.","authors":"Shigetoshi Mizumoto, Yozo Nagao, Hitomi Watanabe, Keiko Tanaka, Takeshi Kuramoto","doi":"10.1016/j.rbmo.2024.104415","DOIUrl":null,"url":null,"abstract":"Research question: What are the clinical effects of using culture media supplemented with antioxidants (AOX) throughout the IVF process?Design: Prospective randomized single-centre study. Cumulus-oocyte complexes and semen samples collected from 127 treatment cycles were divided evenly between the study arm (culture media with AOX) and the control arm (culture media without AOX). The primary endpoint was the good-quality blastocyst (GQB) rate on day 5-6 per metaphase II (MII) oocyte.Results: Fertilization rate and day 5-6 blastocyst rate per MII oocyte differed significantly in favour of the study arm, whereas GQB rate did not. A subgroup analysis, stratified by maternal age, revealed significant improvements in the study arm for day 3 embryo development rate, day 5-6 blastocyst rate, GQB rate and blastocyst utilization rate for patients aged 35-40 years, while the impacts on these endpoints were much smaller in patients aged <35 years. Ninety-four single vitrified blastocyst transfers (SVBT) were performed in each arm. The blastocysts derived from the study arm showed better results of SVBT for patients aged 35-40 years, defined by embryo implantation rate, fetal heartbeat rate and live birth rate, whereas these variables did not differ significantly between the two arms when assessing the results for patients of all ages and patients aged <35 years.Conclusions: Embryo development and SVBT outcomes of treatment cycles of patients aged 35-40 years improved significantly when using AOX-supplemented culture media throughout the IVF process.","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 1","pages":"104415"},"PeriodicalIF":3.7000,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproductive biomedicine online","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.rbmo.2024.104415","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Research question: What are the clinical effects of using culture media supplemented with antioxidants (AOX) throughout the IVF process?

Design: Prospective randomized single-centre study. Cumulus-oocyte complexes and semen samples collected from 127 treatment cycles were divided evenly between the study arm (culture media with AOX) and the control arm (culture media without AOX). The primary endpoint was the good-quality blastocyst (GQB) rate on day 5-6 per metaphase II (MII) oocyte.

Results: Fertilization rate and day 5-6 blastocyst rate per MII oocyte differed significantly in favour of the study arm, whereas GQB rate did not. A subgroup analysis, stratified by maternal age, revealed significant improvements in the study arm for day 3 embryo development rate, day 5-6 blastocyst rate, GQB rate and blastocyst utilization rate for patients aged 35-40 years, while the impacts on these endpoints were much smaller in patients aged <35 years. Ninety-four single vitrified blastocyst transfers (SVBT) were performed in each arm. The blastocysts derived from the study arm showed better results of SVBT for patients aged 35-40 years, defined by embryo implantation rate, fetal heartbeat rate and live birth rate, whereas these variables did not differ significantly between the two arms when assessing the results for patients of all ages and patients aged <35 years.

Conclusions: Embryo development and SVBT outcomes of treatment cycles of patients aged 35-40 years improved significantly when using AOX-supplemented culture media throughout the IVF process.

查看原文本刊更多论文

研究问题：在整个试管婴儿过程中使用添加了抗氧化剂（AOX）的培养基有何临床效果？前瞻性随机单中心研究。将从 127 个治疗周期中收集的积液-卵母细胞复合物和精液样本平均分配给研究组（含 AOX 的培养基）和对照组（不含 AOX 的培养基）。主要终点是第 5-6 天每个分裂期 II（MII）卵母细胞的优质囊胚（GQB）率：结果：受精率和第 5-6 天每个 MII 卵母细胞的囊胚率在研究组中差异显著，而 GQB 率则没有差异。按产妇年龄分层的亚组分析表明，对于 35-40 岁的患者，研究组在第 3 天胚胎发育率、第 5-6 天囊胚率、GQB 率和囊胚利用率方面均有显著改善，而对于年龄在 35-40 岁的患者，这些终点的影响要小得多：在整个体外受精过程中使用添加 AOX 的培养基时，35-40 岁患者治疗周期的胚胎发育和 SVBT 结果均有显著改善。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Reproductive biomedicine online 医学-妇产科学

CiteScore

7.20

自引率

7.50%

发文量

391

审稿时长

50 days

期刊介绍： Reproductive BioMedicine Online covers the formation, growth and differentiation of the human embryo. It is intended to bring to public attention new research on biological and clinical research on human reproduction and the human embryo including relevant studies on animals. It is published by a group of scientists and clinicians working in these fields of study. Its audience comprises researchers, clinicians, practitioners, academics and patients. Context: The period of human embryonic growth covered is between the formation of the primordial germ cells in the fetus until mid-pregnancy. High quality research on lower animals is included if it helps to clarify the human situation. Studies progressing to birth and later are published if they have a direct bearing on events in the earlier stages of pregnancy.