Discovery of LINC01614 associated with the SPP1 gene in colorectal cancer.

IF 2.9 4区医学 Q2 PATHOLOGY

Pathology, research and practice Pub Date : 2024-12-09 DOI:10.1016/j.prp.2024.155761

Fatemeh Norouzinasab, Niloufar Salimian, Khatere Mokhtari, Mohammadarian Akbari, Mazaher Maghsoudloo, Maliheh Entezari, Afshin Taheriazam, Najma Farahani, Mehrdad Hashemi

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引用次数: 0

Abstract

Colorectal cancer (CRC) is a prevalent malignancy worldwide, driven by complex molecular mechanisms. This study aims to elucidate the role of lncRNAs within TGF-β pathway, a crucial signaling pathway in CRC progression, focusing specifically on their interaction with the SPP1 gene. We employed a multi-faceted approach, starting with comprehensive in silico analyses to identify candidate lncRNAs potentially involved in TGF-β pathway regulation. These candidates were further validated through experimental RT-qPCR assays, comparing lncRNA expression profiles in CRC tissues to adjacent normal samples. Our findings revealed novel lncRNA candidates with significant associations with SPP1 in CRC, highlighting their potential regulatory roles in the TGF-β pathway. This integrative study underscores the importance of combining computational predictions with laboratory experimentation to uncover complex regulatory networks in cancer, providing insights into new therapeutic targets and diagnostic biomarkers for CRC.

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结直肠癌中与SPP1基因相关的LINC01614的发现。

结直肠癌（CRC）是一种全球流行的恶性肿瘤，由复杂的分子机制驱动。本研究旨在阐明 lncRNAs 在 TGF-β 通路（CRC 进展的关键信号通路）中的作用，特别关注它们与 SPP1 基因的相互作用。我们采用了一种多方面的方法，首先进行了全面的硅学分析，以确定可能参与TGF-β通路调控的候选lncRNA。通过实验性RT-qPCR测定进一步验证了这些候选lncRNA，比较了CRC组织和邻近正常样本中lncRNA的表达谱。我们的研究结果揭示了在 CRC 中与 SPP1 有显著关联的新型 lncRNA 候选者，突出了它们在 TGF-β 通路中的潜在调控作用。这项综合研究强调了将计算预测与实验室实验相结合以揭示癌症复杂调控网络的重要性，从而为 CRC 的新治疗靶点和诊断生物标志物提供见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pathology, research and practice 医学-病理学

CiteScore

5.00

自引率

3.60%

发文量

405

审稿时长

24 days

期刊介绍： Pathology, Research and Practice provides accessible coverage of the most recent developments across the entire field of pathology: Reviews focus on recent progress in pathology, while Comments look at interesting current problems and at hypotheses for future developments in pathology. Original Papers present novel findings on all aspects of general, anatomic and molecular pathology. Rapid Communications inform readers on preliminary findings that may be relevant for further studies and need to be communicated quickly. Teaching Cases look at new aspects or special diagnostic problems of diseases and at case reports relevant for the pathologist''s practice.