Association of G12D mutation in the KRAS gene with HPV and EBV in gastrointestinal cancer tissues.

IF 1.4 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Journal of International Medical Research Pub Date : 2024-12-01 DOI:10.1177/03000605241302302

Vahideh Hamidi Sofiani, Arefeh Ebrahimian Shiadeh, Alijan Tabarraei, Hadi Razavi Nikoo, Farzin Sadeghi, Ghodsieh Kamrani, Yousef Yahyapour, Abdolvahab Moradi

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引用次数: 0

Abstract

Objective: This study aimed to explore the potential relationship between viral infections and gastrointestinal (GI) malignancies, focusing on the presence of KRAS G12D mutations. Specifically, we investigated the association of viral agents, including human papillomavirus (HPV) and Epstein-Barr virus (EBV), with KRAS G12D mutations in GI cancers to better understand their combined role in cancer development.

Methods: This cross-sectional study comprised 92 patients diagnosed with GI cancer and 100 healthy individuals in the control group. All samples were examined to detect the KRAS G12D gene mutation and the existence of HPV and EBV using real-time polymerase chain reaction assays.

Results: HPV and EBV DNA were detected in 5.4% and 51.4% of gastric cancer samples and in 7.3% and 49.1% of colorectal cancer samples, respectively. Analysis of KRAS G12D in plasma samples revealed heterozygous mutations in 54% of patients with gastric cancer and 35% of patients with colorectal tumors. Among EBV-positive colorectal cancer samples, 1.8% were wild-type, while 47.2% exhibited heterozygous mutations. Among HPV-positive colorectal cancer patients, 1.8% exhibited wild-type KRAS, 5.4% had heterozygous mutations, and 3.2% had homozygous mutations.

Conclusion: This study detected a significant correlation between the presence of viral agents and KRAS G12D mutations.

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胃肠癌组织中 KRAS 基因的 G12D 突变与 HPV 和 EBV 的关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of International Medical Research 医学-药学

CiteScore

3.20

自引率

0.00%

发文量

555

审稿时长

1 months

期刊介绍： _Journal of International Medical Research_ is a leading international journal for rapid publication of original medical, pre-clinical and clinical research, reviews, preliminary and pilot studies on a page charge basis. As a service to authors, every article accepted by peer review will be given a full technical edit to make papers as accessible and readable to the international medical community as rapidly as possible. Once the technical edit queries have been answered to the satisfaction of the journal, the paper will be published and made available freely to everyone under a creative commons licence. Symposium proceedings, summaries of presentations or collections of medical, pre-clinical or clinical data on a specific topic are welcome for publication as supplements. Print ISSN: 0300-0605