Celine Kelso, Alan T Maccarone, Anton I P M de Kroon, Todd W Mitchell, Mike F Renne
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引用次数: 0
Abstract
Yeast is a poikilothermic organism and adapts its lipid composition to the environmental temperature to maintain membrane physical properties. Studies addressing temperature-dependent adaptation of the lipidome have described changes in the phospholipid composition at the level of sum composition (e.g. PC 32:1) and molecular composition (e.g. PC 16:0_16:1). However, there is little information at the level of positional isomers (e.g. PC 16:0/16:1 versus PC 16:1/16:0). Here, we used collision- and ozone-induced dissociation (CID/OzID) mass spectrometry to investigate homeoviscous adaptation of PC, PE and PS to determine the phospholipid acyl chains at the sn-1 and sn-2 position. Our data establish the sn-molecular species composition of PC, PE and PS in the lipidome of yeast cultured at different temperatures.
酵母是一种变温生物，它会根据环境温度调整其脂质组成，以保持膜的物理特性。针对脂质体温度适应性的研究描述了磷脂组成在总组成（如 PC 32:1）和分子组成（如 PC 16:0_16:1）层面的变化。然而，位置异构体（如 PC 16:0/16:1 与 PC 16:1/16:0）层面的信息却很少。在此，我们利用碰撞和臭氧诱导解离（CID/OzID）质谱法研究了 PC、PE 和 PS 的顺粘适应性，以确定 sn-1 和 sn-2 位置的磷脂酰基链。我们的数据确定了在不同温度下培养的酵母脂质体中 PC、PE 和 PS 的 sn 分子物种组成。
期刊介绍：
FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.
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