Multi-sample analytical workflow for the determination of isoprostanes in oral fluid: A new tool for non-invasive evaluation of oxidative stress.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-04-01 Epub Date: 2024-12-09 DOI:10.1016/j.talanta.2024.127358

Francesco Bartolini, Martina Croce, Francesco Della Valle, Camilla Montesano, Federico Fanti, Dario Compagnone, Manuel Sergi

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引用次数: 0

Abstract

Oxidative stress is a pathological condition that contributes to the onset of various diseases. In this way, studying oxidative stress could lead to significant discoveries in the field of therapeutic and preventive medicine. Lipid peroxidation is the most significant event in the oxidative stress process and the gold standard biomarkers for endogenous oxidative damage to lipids are isoprostanes (IsoPs). This project aims to develop a reliable analytical method for the liquid-liquid microextraction technique, parallel artificial liquid membrane extraction (PALME) and LC-MS/MS analysis. PALME allowed to obtain a significant enrichment factor and, at the same time, a good sample purification by removing compounds that cause signal suppression, thereby reducing matrix effect. The chromatographic and mass spectrometric conditions have been fine tuned to improve the sensitivity of the method and therefore obtaining very low LOD and LOQ values. The recovery values obtained for the analytes are slightly above 50 %, except for 6-keto Prostaglandin F1A (24 %). Matrix effects were ≤ -10 %, with LODs ranging between 1 and 5 pg mL^-1. The developed method is characterized by high sensitivity and low consumption of organic solvents, according to the principles of Green Analytical Chemistry and enables the determination of basal levels of IsoPs in oral fluid by processing 96 samples simultaneously.

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测定口腔液中异前列腺素的多样品分析工作流程：无创评估氧化应激的新工具。

氧化应激是一种病理状态，有助于各种疾病的发病。通过这种方式，研究氧化应激可以在治疗和预防医学领域带来重大发现。脂质过氧化是氧化应激过程中最重要的事件，而内源性脂质氧化损伤的金标准生物标志物是异prostanes （IsoPs）。本项目旨在建立一种可靠的液液微萃取技术、平行人工液膜萃取（PALME）和LC-MS/MS分析的分析方法。PALME允许获得显著的富集因子，同时，通过去除引起信号抑制的化合物，从而降低基质效应，实现了良好的样品纯化。对色谱和质谱条件进行了微调，以提高方法的灵敏度，从而获得非常低的LOD和LOQ值。除6-酮前列腺素F1A（24%）外，分析物的回收率略高于50%。基质效应≤- 10%，lod范围为1 ~ 5 pg mL-1。该方法根据绿色分析化学原理，具有灵敏度高、有机溶剂消耗少的特点，可同时处理96个样品，测定口服液中IsoPs的基础水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.